ArticleInternational journal of clinical and experimental pathology2017
Hydroxyapatite nanocrystals stimulate osteogenic differentiation in primary human aortic smooth muscle cells by activation of oxidative stress and the ERK pathway.
Article in International journal of clinical and experimental pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- Comparative study of bovine and synthetic hydroxyapatite in micro- and nanosized on osteoblasts action and bone growth.PloS one · 2025Article
- Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial.Pharmaceuticals (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHydroxyapatite nanocrystal (HN) deposition underlies the development of vascular calcification, which is an actively regulated process resembling bone formation. This study investigated the role of HNs in inducing osteogenic differentiation of primary human aortic smooth muscle cells (HASMCs).
methodsPrimary HASMCs were incubated with HNs, cell osteogenic differentiation was evaluated by von kossa staining and calcium content. The expressions of SM-α-actin and bone markers, including runt-related transcription factor 2 (Runx2), osteopontin (OPN), osterix, and collagen 1 (COL1) were also determined. Antioxidants, ERK-specific inhibitor were used to examine whether oxidative stress and the ERK pathway were required for this transition.
resultsStimulation of HASMCs with HNs increased calcium deposition, expression of bone markers and decreased SM-α-actin expression. HNs produced reactive oxygen species (ROS) in HASMCs, as evaluated by fluorescent probe. Antioxidants inhibited HN-induced osteogenic differentiation. Furthermore, the inhibitor of the ERK pathway, PD98059, suppressed the effect of HNs on bone marker expression.
conclusionsThese findings suggest that HNs stimulated osteogenic differentiation of vascular smooth muscle cells that build biomineralized deposits partly by activating oxidative stress and the ERK pathway.
Indexed as
Identifiers
31966619PMC6965225W3027795762What OpenQuestion holds
Registered trials
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