Evidence map›Paper›PMID 31966401›Full record

ArticleInternational journal of clinical and experimental pathology2017

Inhibitory effects of pigment epithelium-derived factor on epithelial-mesenchymal transition, migration and invasion of breast cancer.

Dan Zhou, Pengcheng Xu, Min Zhang, Guolin Ye, Lin Zhang

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 75% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Dan ZhouDepartment of Breast Surgery, Foshan Hospital Affiliated to Sun Yat-Sen University, The First People's Hospital of Foshan City Foshan 528100, Guangdong, P.R. China.
Pengcheng XuDepartment of Histology and Embryology, State Key Laboratory, Southern Medical University Guangzhou 510515, Guangdong, P.R. China.
Min ZhangDepartment of Histology and Embryology, State Key Laboratory, Southern Medical University Guangzhou 510515, Guangdong, P.R. China.
Guolin YeDepartment of Breast Surgery, Foshan Hospital Affiliated to Sun Yat-Sen University, The First People's Hospital of Foshan City Foshan 528100, Guangdong, P.R. China.
Lin ZhangDepartment of Histology and Embryology, State Key Laboratory, Southern Medical University Guangzhou 510515, Guangdong, P.R. China.
Southern Medical University · CNFirst People's Hospital of Foshan · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivePigment epithelium-derived factor (PEDF) is a ubiquitously expressed secreted protein that suppresses tumor growth and metastasis by targeting tumor cells and their microenvironment. However, the exact mechanism of PEDF on breast cancer metastasis including liver and lung metastasis remains unclear. Epithelial-mesenchymal transition (EMT) is a pivotal event in the progression of cancer towards metastasis. In the present report, we investigated whether PEDF inhibits breast cancer metastasis through epithelial-mesenchymal transition and elucidate the association of PEDF expression and EMT in vitro.

methodsOur analyses were performed on 102 tissue samples of patients with primary BC and a set of 20 control samples of healthy women, respectively. Lentiviruses were used to stably express PEDF in SkBr3 breast cancer cell line to determine EMT factors changes of invasion ability following PEDF re-expression. PEDF and EMT factors protein levels were measured in SkBr3 breast cancer cell line using western blot analyses.

resultsWe show that the important inhibitor of angiogenesis, pigment epithelium-derived factor expression positively correlatedwith lymph node-positive tumor status and tumor size, low expression level of vimentin, and high expression levels of membranous E-cadherin. In addition, we found that PEDF activation suppressed migration and invasion in SKBR3 (luminal) cellsand led to morphologic and molecular changes of epithelial-mesenchymal transition (EMT). Loss of PEDF promotesmesenchymal phenotype, whereas PEDF was shown to effectively promotes epithelial phenotyperesulted ininhibited the growthof endocrine-resistant SkBr3 breast cancer cells invitro. Finally, western blot examination of PEDF/siRNA-expressing tumor showed down-regulation of E-cadherin and up-regulation of vimentin.

conclusionsThese findings suggest that PEDF is directly linked to the mechanisms that suppress metastasis of breast cancer through regulating epithelial-mesenchymal transition. In the future, contribute to evaluate the efficacy of PEDF targetedtherapy early during the course of the disease, may be beneficial in the treatment of breast cancer patients.

Indexed as

Breast cancerepithelial-mesenchymal transitionmetastasispigment epithelium-derived factor

Identifiers

PMID31966401
PMCPMC6965765
OpenAlexW3012138177

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.