Evidence map›Paper›PMID 31965190›Full record

ArticleIntegrative biology : quantitative biosciences from nano to macro2020

Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential.

Efraín A Cermeño, Meghan J O'Melia, Woojin M Han, Austin Veith, Graham Barber, Emina H Huang, Susan N Thomas, Andrés J García

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Integrative biology : quantitative biosciences from nano to macro, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Efraín A CermeñoWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Meghan J O'MeliaPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.
Woojin M HanWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Austin VeithWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Graham BarberWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Emina H HuangLerner Research Institute, Department of Cancer Biology, Department of Colorectal Surgery, Cleveland Clinic, Cleveland, OH, USA.
Susan N ThomasWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Andrés J GarcíaWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Georgia Institute of Technology · USCleveland Clinic Lerner College of Medicine · USThe Wallace H. Coulter Department of Biomedical Engineering · US

Funding

Research Training Program Plan on Cell and Tissue Engineering (CTEng)T32GM008433 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI GARCIA, ANDRES J · 1991 to 2021
$7.1M
An organotypic model recapitulating colon cancer microenvironment and metastasisU01CA214300 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI HUANG, EMINA HUI-NA, SHEN, XILING · 2017 to 2021
$3.1M
The miR-20/c-Myc/E2F Regulatory Axis is Critical for the Tumor Promoting Activity of Inflammatory Fibroblasts in Colitis-Associated CancerR01CA237304 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI HUANG, EMINA HUI-NA, WESSELY, OLIVER · 2019 to 2023
$2.9M
uSHEAR Technology for Cancer Cell PurificaitonR21CA202849 · NCI · GEORGIA INSTITUTE OF TECHNOLOGY · PI GARCIA, ANDRES J, THOMAS, SUSAN NAPIER · 2016 to 2018
$600k
NCI NIH HHS R01 CA237304NCI NIH HHS R21 CA202849NCI NIH HHS U01 CA214300NIGMS NIH HHS T32 GM008433
6 · The paper itself

Abstract

Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.

Indexed as

Cell AdhesionHydrodynamicsAnimalsBreast NeoplasmsCarcinogenesisCell Line, TumorCell SeparationDisease ProgressionFemaleGreen Fluorescent ProteinsHumansMCF-7 CellsMiceMice, Inbred C57BLMice, SCIDMicrofluidicsGreen Fluorescent Proteinscancercell adhesionhydrodynamicmicrofluidictumor-initiating cells

Identifiers

PMID31965190
PMCPMC7036474
OpenAlexW3002974744

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.