ArticleNature protocols2020
Isolation of satellite cells and transplantation into mice for lineage tracing in muscle.
Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Bioinspired hydrogel-based regenerative engineering strategies for the structural and functional regeneration of skeletal muscles following volumetric muscle loss.Regenerative biomaterials · 2026Review
- Lineage relationships in muscle stem cells: linking functional fate to molecular state.Frontiers in cell and developmental biology · 2026Review
- TBC1D1 functions as a negative regulator of satellite cells for muscle regeneration.Nature communications · 2025Article
- Nuclear entry of AS160 as a transcriptional regulator of satellite cells for muscle regeneration.Nature communications · 2025Article
- Rapamycin improves satellite cells' autophagy and muscle regeneration during hypercapnia.JCI insight · 2025Article
- Combined magnesium and silicon ions synergistically promote functional regeneration of skeletal muscle by regulating satellite cell fate.Regenerative biomaterials · 2025Article
- Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells.Bio-protocol · 2024Article
- Resistance training does not increase myocellular garbage dumps: A pilot study on lipofuscin in skeletal muscle fibers of resistance trained young men.Physiological reports · 2024Article
- Single-cell chromatin accessibility profiling reveals a self-renewing muscle satellite cell state.The Journal of cell biology · 2023Article
- Resistance and endurance exercise training improves muscle mass and the inflammatory/fibrotic transcriptome in a rhabdomyosarcoma model.Journal of cachexia, sarcopenia and muscle · 2023Article
- Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Impaired regenerative capacity contributes to skeletal muscle dysfunction in chronic obstructive pulmonary disease.American journal of physiology. Cell physiology · 2022Review
- Deaccelerated Myogenesis and Autophagy in Genetically Induced Pulmonary Emphysema.American journal of respiratory cell and molecular biology · 2022Article
- Lipid droplet dynamics regulate adult muscle stem cell fate.Cell reports · 2022Article
- Transplantation to study satellite cell heterogeneity in skeletal muscle.Frontiers in cell and developmental biology · 2022Review
- Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.Developmental cell · 2021Article
- Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers.Skeletal muscle · 2021Article
- Enhancing Interrogation of Skeletal Muscle Samples for Informative Quantitative Data.Journal of neuromuscular diseases · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Limited methods exist to assay the direct effects of therapeutic intervention on muscle stem cell fate, proliferation or differentiation in an in vivo context. Here we provide an optimized protocol for muscle stem cell isolation and transplantation into mice to deconvolute heterogeneity within isolated stem cell populations. Viable and pure cell populations are isolated within 2 h and can then be used for therapeutic intervention or transplantation to uncover the repopulating and differentiation potential in mice, a physiologically relevant in vivo context. Effects can be assessed 9 d after transplantation. This methodology analyzes cell and sort purity prior to transplantation to improve reproducibility and outlines novel blocking steps to improve tissue staining and analysis. Experience with surgical procedures in mice is recommended before attempting this protocol. Our system is widely applicable for exploring stem cell dynamics within muscle and has already been used to study heterogeneity within muscle stem cell populations and efficacy of therapeutic intervention on isolated stem cell populations.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.