ArticleScientific reports2020
Describing the fecal metabolome in cryogenically collected samples from healthy participants.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Milk polar lipids favorably alter circulating and intestinal ceramide and sphingomyelin species in postmenopausal women.JCI insight · 2021Trial
- The faecal bulk heterogeneity: implications for homogenisation and spot-sampling strategies for metabolomic investigations.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Microbiota and lipid mediators: from molecular crosstalk to therapeutic opportunities.Frontiers in pharmacology · 2026Review
- Development of a multi-targeted metabolomics platform for semi-quantification of faecal metabolites: a proof-of-concept analysis in human faeces.Microbiome research reports · 2026Article
- Development of an untargeted metabolomics analytical protocol for fecal samples by liquid chromatography-mass spectrometry.Journal of food and drug analysis · 2025Article
- Evaluation of inter- and intra-variability in gut health markers in healthy adults using an optimised faecal sampling and processing method.Scientific reports · 2024Article
- Plasma cathepsin D as an early indicator of alcohol-related liver disease.JHEP reports : innovation in hepatology · 2024Article
- Comparative Metabolomics and Microbiome Analysis of Ethanol versus OMNImet/gene•GUT Fecal Stabilization.Analytical chemistry · 2024Article
- Article
- Linking Gut Microbiome and Lipid Metabolism: Moving beyond Associations.Metabolites · 2021Review
- Effect of Dietary Protein Levels on Dynamic Changes and Interactions of Ruminal Microbiota and Metabolites in Yaks on the Qinghai-Tibetan Plateau.Frontiers in microbiology · 2021Article
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Authors and funding
12 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chemical composition of feces plays an important role in human metabolism. Metabolomics and lipidomics are valuable tools for screening the metabolite composition in feces. Here we set out to describe fecal metabolite composition in healthy participants in frozen stools. Frozen stool samples were collected from 10 healthy volunteers and cryogenically drilled in four areas along the specimen. Polar metabolites were analyzed using derivatization followed by two-dimensional gas chromatography and time of flight mass spectrometry. Lipids were detected using ultra high-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. 2326 metabolic features were detected. Out of a total of 298 metabolites that were annotated we report here 185 that showed a technical variation of x < 30%. These metabolites included amino acids, fatty acid derivatives, carboxylic acids and phenolic compounds. Lipids predominantly belonged to the groups of diacylglycerols, triacylglycerols and ceramides. Metabolites varied between sampling areas, some were broadly homogeneous, others varied 80%. A LASSO-computed network using metabolites present in all areas showed two main clusters describing the system, DAG lipids and phenyllactic acid. In feces from healthy participants, the main groups detected were phenolic compounds, ceramides, diacylglycerols and triacylglycerols.
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