ArticleDevelopment (Cambridge, England)2020
Single-cell RNAseq analysis of testicular germ and somatic cell development during the perinatal period.
Article in Development (Cambridge, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed.
- Article
- RUNX proteins and CBFβ create an interconnected network of transcriptional regulation in the male germline†.Biology of reproduction · 2026Article
- Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate.Cell reports · 2026Article
- Identification of genes differentially expressed in granulosa cells from women with high vs. low ovarian responsiveness.Frontiers in reproductive health · 2026Article
- Differential retinoic acid responses across testicular developmentbioRxiv : the preprint server for biology · 2025Article
- Nicheformer: a foundation model for single-cell and spatial omics.Nature methods · 2025Article
- Testicular stage- and cell-specific expression of F-actin-binding proteins†.Biology of reproduction · 2025Article
- A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular Niche.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- An ancient enhancer rapidly evolving in the human lineage promotes neural development and cognitive flexibility.Science advances · 2025Article
- Review
- Article
- Cell type- and factor-specific nonsense-mediated RNA decay.Nucleic acids research · 2025Article
- Functional Analysis of HSD17B3-Deficient Male Mice Reveals Roles for HSD17B7 and HSD17B12 in Testosterone Biosynthesis.Endocrinology · 2025Article
- Article
- Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells.Cell discovery · 2025Article
- Single-Cell RNA Sequencing Reveals an Atlas of Meihua Pig Testis Cells.Animals : an open access journal from MDPI · 2025Article
- Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice.Cell proliferation · 2025Article
- Insights from the single-cell level: lineage trajectory and somatic-germline interactions during spermatogenesis in dwarf surfclam Mulinia lateralis.BMC genomics · 2025Article
- Transcriptomic dynamics and cell-to-cell communication during the transition of prospermatogonia to spermatogonia revealed at single-cell resolution.BMC genomics · 2025Article
- Sertoli cells as a hub in testicular development and male reproductive.Frontiers in cell and developmental biology · 2025Review
15 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Pro-spermatogonia (SG) serve as the gateway to spermatogenesis. Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants and testicular somatic cells during the perinatal period in mice. We identified both gene and protein markers for three temporally distinct ProSG cell subsets, including a migratory cell population with a transcriptome distinct from the previously defined T1- and T2-ProSG stages. This intermediate (I)-ProSG subset translocates from the center of seminiferous tubules to the spermatogonial stem cell (SSC) 'niche' in its periphery soon after birth. We identified three undifferentiated SG subsets at postnatal day 7, each of which expresses distinct genes, including transcription factor and signaling genes. Two of these subsets have the characteristics of newly emergent SSCs. We also molecularly defined the development of Sertoli, Leydig and peritubular myoid cells during the perinatal period, allowing us to identify candidate signaling pathways acting between somatic and germ cells in a stage-specific manner during the perinatal period. Our study provides a rich resource for those investigating testicular germ and somatic cell developmental during the perinatal period.
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