ArticleJournal of lipid research2020
An LC/MS/MS method for analyzing the steroid metabolome with high accuracy and from small serum samples.
Article in Journal of lipid research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- A simultaneous quantification method of 22 steroid hormones in human serum based on PP-SPE and ID-LC-MS/MS.Analytical and bioanalytical chemistry · 2026Article
- Highly specific ID-UHPLC-MS/MS method for analyzing polar and non-polar steroid hormones.Analytical and bioanalytical chemistry · 2026Article
- Steroid Fingerprinting with Cryogenic Gas-Phase Infrared Spectroscopy.ACS measurement science au · 2026Article
- Differentiation of steroid isomers via derivatization and electron-activated dissociation tandem mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- Adulteration of Sports Supplements with Anabolic Steroids-From Innocent Athlete to Vicious Cheater.Nutrients · 2025Review
- Steroid dynamics in myalgic encephalomyelitis / chronic fatigue syndrome: a case-control study using ultra performance supercritical fluid chromatography tandem mass spectrometry.Journal of translational medicine · 2025Article
- Mass-spectrometry based metabolomics: an overview of workflows, strategies, data analysis and applications.Proteome science · 2025Review
- Advances in steroid purification for novel techniques in carbon isotope ratio mass spectrometry of doping control.RSC advances · 2025Review
- Quantitative analysis of cannabinoids and metabolites in oral fluid by volumetric absorptive microsampling combined with UHPLC-HRMS.Analytical and bioanalytical chemistry · 2025Article
- Case Report: Trilostane therapy in a dog with recurrent adrenocortical carcinoma producing an array of steroid hormones.Frontiers in veterinary science · 2025Article
- Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.ACS chemical neuroscience · 2024Article
- In vitro comparative study of multimodal imaging nano-assembled microspheres with two clinical drug-eluting beads loaded with doxorubicin.Drug delivery · 2023Article
- Sterol and lipid metabolism in bees.Metabolomics : Official journal of the Metabolomic Society · 2023Review
- Elevated follicular cortisone level is a negative predictor of clinical pregnancy in women undergoing fresh embryo transfer.Heliyon · 2023Article
- GC-MS/MS Determination of Steroid Hormones in Urine Using Solid-Phase Derivatization as an Alternative to Conventional Methods.Molecules (Basel, Switzerland) · 2022Article
- Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples.Metabolites · 2022Article
- Metabolic Alteration Analysis of Steroid Hormones in Niemann-Pick Disease Type C Model Cell Using Liquid Chromatography/Tandem Mass Spectrometry.International journal of molecular sciences · 2022Article
- Effect of maternal body mass index on the steroid profile in women with gestational diabetes mellitus.Frontiers in endocrinology · 2022Article
- Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis.Journal of lipid research · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analyzing global steroid metabolism in humans can shed light on the etiologies of steroid-related diseases. However, existing methods require large amounts of serum and lack the evaluation of accuracy. Here, we developed an LC/MS/MS method for the simultaneous quantification of 12 steroid hormones: testosterone, pregnenolone, progesterone, androstenedione, corticosterone, 11-deoxycortisol, cortisol, 17-hydroxypregnenolone, 17-hydroxyprogesterone, dehydroepiandrosterone, estriol, and estradiol. Steroids and spiked internal standards in 100 μl serum were extracted by protein precipitation and liquid-liquid extraction. The organic phase was dried by evaporation, and isonicotinoyl chloride was added for steroid derivatization, followed by evaporation under nitrogen and redissolution in 50% methanol. Chromatographic separation was performed on a reverse-phase PFP column, and analytes were detected on a triple quadrupole mass spectrometer with ESI. The lower limits of quantification ranged from 0.005 ng/ml for estradiol to 1 ng/ml for cortisol. Apparent recoveries of steroids at high, medium, and low concentrations in quality control samples were between 86.4% and 115.0%. There were limited biases (-10.7% to 10.5%) between the measured values and the authentic values, indicating that the method has excellent reliability. An analysis of the steroid metabolome in pregnant women highlighted the applicability of the method in clinical serum samples. We conclude that the LC/MS/MS method reported here enables steroid metabolome analysis with high accuracy and reduced serum consumption, indicating that it may be a useful tool in both clinical and scientific laboratory research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.