ArticleAnimals : an open access journal from MDPI2020
Measurement of Fecal Testosterone Metabolites in Mice: Replacement of Invasive Techniques.
Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Colour Morphs as Alternative Solutions to the Trade-Off Predicted by the Immuno-Competence Handicap Hypothesis.Integrative zoology · 2026Article
- Anti-Müllerian Hormone as a Marker for Castration Status and Fertility in Bulls and Oxen.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Circadian rhythms in sexual behavior and their influence on reproductive outcomes in mice.bioRxiv : the preprint server for biology · 2025Article
- Acute stress causes sex-specific changes to ventral subiculum synapses, circuitry, and anxiety-like behavior.Nature communications · 2025Article
- Comparison of hair steroid levels among Wistar rats exposed to different environmental enrichment settings.Veterinary world · 2024Article
- Role of intestinal testosterone-degrading bacteria and 3/17β-HSD in the pathogenesis of testosterone deficiency-induced hyperlipidemia in males.NPJ biofilms and microbiomes · 2024Article
- Validation of a triiodothyronine (T3) ELISA for mouse fecal samples.Physiological reports · 2024Article
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- Gut microbiota is involved in male reproductive function: a review.Frontiers in microbiology · 2024Review
- Guidelines on the use of sex and gender in cardiovascular research.American journal of physiology. Heart and circulatory physiology · 2024Review
- A time for sex: circadian regulation of mammalian sexual and reproductive function.Frontiers in neuroscience · 2024Review
- Non-Invasive Quantification of Faecal and Urine Reproductive Hormone Metabolites in the Naked Mole-Rat (Animals : an open access journal from MDPI · 2023Article
- Generalizability, Robustness and Replicability When Evaluating Wellbeing of Laboratory Mice with Various Methods.Animals : an open access journal from MDPI · 2022Article
- Sex Differences in Pubertal Circadian and Ultradian Rhythmic Development Under Semi-naturalistic Conditions.Journal of biological rhythms · 2022Article
- Mutual Shaping of Circadian Body-Wide Synchronization by the Suprachiasmatic Nucleus and Circulating Steroids.Frontiers in behavioral neuroscience · 2022Review
- Adolescent Development of Biological Rhythms in Female Rats: Estradiol Dependence and Effects of Combined Contraceptives.Frontiers in physiology · 2021Article
- Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice.Animals : an open access journal from MDPI · 2020Article
- Improving reproducibility in animal research by splitting the study population into several 'mini-experiments'.Scientific reports · 2020Article
- Social enrichment by separated pair housing of male C57BL/6JRj mice.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Testosterone is the main reproductive hormone in male vertebrates and conventional methods to measure testosterone rely on invasive blood sampling procedures. Here, we aimed to establish a non-invasive alternative by assessing testosterone metabolites (TMs) in fecal and urinary samples in mice. We performed a radiometabolism study to determine the effects of daytime and sex on the metabolism and excretion pattern of radiolabeled TMs. We performed physiological and biological validations of the applied EIA to measure TMs and assessed diurnal fluctuations in TM excretions in male and female mice and across strains. We found that males excreted significantly more radiolabeled TMs via the feces (59%) compared to females (49.5%). TM excretion patterns differed significantly between urinary and fecal samples and were affected by the daytime of ³H-testosterone injection. Overall, TM excretion occurred faster in urinary than fecal samples. Peak excretion of fecal TMs occurred after 8 h when animals received the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.