Evidence map›Paper›PMID 31963386›Full record

ArticleMicroorganisms2020

Nuclear Gene Transformation in the Dinoflagellate

Brittany N Sprecher, Huan Zhang, Senjie Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. RNA-Binding Proteins in Dinoflagellates.International journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Brittany N SprecherDepartment of Marine Sciences, University of Connecticut, 1080 Shennecossett Rd, Groton, CT 06340, USA.ORCID 0000-0001-5032-3834
Huan ZhangDepartment of Marine Sciences, University of Connecticut, 1080 Shennecossett Rd, Groton, CT 06340, USA.
Senjie LinDepartment of Marine Sciences, University of Connecticut, 1080 Shennecossett Rd, Groton, CT 06340, USA.ORCID 0000-0001-8831-6111
University of Connecticut · US

Funding

Gordon and Betty Moore Foundation GBMF4980National Science Foundation 1247393
6 · The paper itself

Abstract

The lack of a robust gene transformation tool that allows proper expression of foreign genes and functional testing for the vast number of nuclear genes in dinoflagellates has greatly hampered our understanding of the fundamental biology in this ecologically important and evolutionarily unique lineage of microeukaryotes. Here, we report the development of a dinoflagellate expression vector containing various DNA elements from phylogenetically separate dinoflagellate lineages, an electroporation protocol, and successful expression of introduced genes in an early branching dinoflagellate,

Indexed as

dinoflagellatesgene transformationmarine biologymolecular biology

Identifiers

PMID31963386
PMCPMC7022241
OpenAlexW3000298078

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.