ArticleThe Journal of experimental medicine2020
The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.
Article in The Journal of experimental medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Interplay of autophagy and Th1/Th2-mediated macrophage polarization in host-pathogen dynamics.Frontiers in cellular and infection microbiology · 2025Pooled it
- Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum.Parasites & vectors · 2025Article
- Defining neuronal responses to the neurotropic parasitemSphere · 2025Article
- The microneme protein1 (MIC1) of Chinese 1 Toxoplasma regulates pyroptosis through the TLR4/NLRP3 pathway in macrophages.Parasites & vectors · 2024Article
- Eimeria: Navigating complex intestinal ecosystems.PLoS pathogens · 2024Review
- Transcriptional profiles analysis of effects ofFrontiers in cellular and infection microbiology · 2024Article
- High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription.Cell host & microbe · 2023Article
- ROP16 ofCells · 2023Article
- ROP16-mediated activation of STAT6 enhances cyst development of type III Toxoplasma gondii in neurons.PLoS pathogens · 2023Article
- Overview of Apoptosis, Autophagy, and Inflammatory Processes inPathogens (Basel, Switzerland) · 2023Review
- Variation in CD8 T cell IFNγ differentiation to strains ofFrontiers in cellular and infection microbiology · 2023Article
- Effects of Ovine Monocyte-Derived Macrophage Infection by Recently IsolatedAnimals : an open access journal from MDPI · 2022Article
- Virulence-related gene wx2 of Toxoplasma gondii regulated host immune response via classic pyroptosis pathway.Parasites & vectors · 2022Article
- Toxoplasmosis vaccines: what we have and where to go?NPJ vaccines · 2022Review
- The hypoxia-driven crosstalk between tumor and tumor-associated macrophages: mechanisms and clinical treatment strategies.Molecular cancer · 2022Review
- Anti-infection roles of miR-155-5p packaged in exosomes secreted by dendritic cells infected with Toxoplasma gondii.Parasites & vectors · 2022Article
- Intestinal immune responses to commensal and pathogenic protozoa.Frontiers in immunology · 2022Review
- Parasitomimetics: Can We Utilize Parasite-Derived Immunomodulatory Molecules for Interventions to Immunological Disorders?Frontiers in immunology · 2022Review
- Toxoplasmosis: Current and Emerging Parasite Druggable Targets.Microorganisms · 2021Review
- Lessons from Toxoplasma: Host responses that mediate parasite control and the microbial effectors that subvert them.The Journal of experimental medicine · 2021Review
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13 authors at 3 institutions in 2 countries.
Funding
Abstract
The ability of Toxoplasma gondii to inject the rhoptry kinase ROP16 into host cells results in the activation of the transcription factors STAT3 and STAT6, but it is unclear how these events impact infection. Here, parasites that inject Cre-recombinase with rhoptry proteins were used to distinguish infected macrophages from those only injected with parasite proteins. Transcriptional profiling revealed that injection of rhoptry proteins alone was sufficient to induce an M2 phenotype that is dependent on STAT3 and STAT6, but only infected cells displayed reduced expression of genes associated with antimicrobial activity and protective immunity. In vivo, the absence of STAT3 or STAT6 improved parasite control, while the loss of ROP16 resulted in a marked reduction in parasite numbers and heightened parasite-specific T cell responses. Thus, ROP16 is a virulence factor that can act in cis and trans to promote M2 programs and which limits the magnitude of parasite-specific T cell responses.
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