ArticlePLoS pathogens2020
G protein α subunit suppresses sporangium formation through a serine/threonine protein kinase in Phytophthora sojae.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 19 citations in OpenAlex.
- Transcriptional programs controlling sporulation in Phytophthora respond to nutrition, humidity, and cryptochrome-mediated light signals.PLoS pathogens · 2026Article
- A conserved Phytophthora apoplastic trypsin-like serine protease targets the receptor-like kinase BAK1 to dampen plant immunity.Nature plants · 2025Article
- Protoplast Transformation of Phytophthora spp.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production ofJournal of fungi (Basel, Switzerland) · 2024Article
- A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription.PLoS pathogens · 2024Article
- PlAtg8-mediated autophagy regulates vegetative growth, sporangial cleavage, and pathogenesis inMicrobiology spectrum · 2024Article
- A Phytophthora receptor-like kinase regulates oospore development and can activate pattern-triggered plant immunity.Nature communications · 2023Article
- A Novel FYVE Domain-Containing Protein Kinase, PsZFPK1, Plays a Critical Role in Vegetative Growth, Sporangium Formation, Oospore Production, and Virulence inJournal of fungi (Basel, Switzerland) · 2023Article
- Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes.PLoS pathogens · 2023Article
- Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development ofJournal of fungi (Basel, Switzerland) · 2023Article
- Signal and regulatory mechanisms involved in spore development ofFrontiers in microbiology · 2022Review
- Specific interaction of an RNA-binding protein with the 3'-UTR of its target mRNA is critical to oomycete sexual reproduction.PLoS pathogens · 2021Article
- Article
- A CRISPR/Cas9-mediated in situ complementation method for Phytophthora sojae mutants.Molecular plant pathology · 2021Article
- A Patched-Like Protein PsPTL Is Not Essential for the Growth and Response to Various Stresses inFrontiers in microbiology · 2021Article
- TOR Inhibitors Synergistically Suppress the Growth and Development ofFrontiers in microbiology · 2021Article
- Organize, Don't Agonize: Strategic Success ofMicroorganisms · 2020Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic heterotrimeric guanine nucleotide-binding proteins consist of α, β, and γ subunits, which act as molecular switches to regulate a number of fundamental cellular processes. In the oomycete pathogen Phytophthora sojae, the sole G protein α subunit (Gα; encoded by PsGPA1) has been found to be involved in zoospore mobility and virulence, but how it functions remains unclear. In this study, we show that the Gα subunit PsGPA1 directly interacts with PsYPK1, a serine/threonine protein kinase that consists of an N-terminal region with unknown function and a C-terminal region with a conserved catalytic kinase domain. We generated knockout and knockout-complemented strains of PsYPK1 and found that deletion of PsYPK1 resulted in a pronounced reduction in the production of sporangia and oospores, in mycelial growth on nutrient poor medium, and in virulence. PsYPK1 exhibits a cytoplasmic-nuclear localization pattern that is essential for sporangium formation and virulence of P. sojae. Interestingly, PsGPA1 overexpression was found to prevent nuclear localization of PsYPK1 by exclusively binding to the N-terminal region of PsYPK1, therefore accounting for its negative role in sporangium formation. Our data demonstrate that PsGPA1 negatively regulates sporangium formation by repressing the nuclear localization of its downstream kinase PsYPK1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.