ArticlePLoS neglected tropical diseases2020
Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Emerging threat of Oropouche virus: Clinical features, experimental models, and virulence.Virulence · 2026Review
- Enhanced virulence and neuroinvasion of contemporaryFrontiers in microbiology · 2026Article
- Viral Metagenomic Next-Generation Sequencing for One Health Discovery and Surveillance of (Re)Emerging Viruses: A Deep Review.International journal of molecular sciences · 2025Review
- Oropouche virus efficiently replicates and is immunostimulatory in vivo in nonhuman primate species.Science advances · 2025Article
- Protein-based tools for the detection and characterisation of Oropouche virus infection.EMBO molecular medicine · 2025Article
- Mysterious Oropouche virus: Transmission, symptoms, and control.Infectious medicine · 2025Review
- Review
- Re-emergence of Oropouche virus as a novel global threat.Current research in microbial sciences · 2025Review
- Genomic Epidemiology of 2023-2024 Oropouche Outbreak in Iquitos, Peru reveals independent origin from a concurrent outbreak in Brazil.medRxiv : the preprint server for health sciences · 2024Article
- Oropouche virus: A re-emerging arbovirus of clinical significance.IJID regions · 2024Article
- Full Genome Characterization of the First Oropouche Virus Isolate Imported in Europe from Cuba.Viruses · 2024Article
- Oropouche Virus: An Emerging Orthobunyavirus.The Journal of general virology · 2024Review
- Epidemiological and clinical overview of the 2024 Oropouche virus disease outbreaks, an emerging/re-emerging neurotropic arboviral disease and global public health threat.Journal of medical virology · 2024Article
- Transmission risk of Oropouche fever across the Americas.Infectious diseases of poverty · 2023Article
- A Chikungunya Outbreak in a Dengue-endemic Region in Rural Northern Coastal Ecuador.The American journal of tropical medicine and hygiene · 2022Article
- Baseline mapping of Oropouche virology, epidemiology, therapeutics, and vaccine research and development.NPJ vaccines · 2022Review
- Oropouche virus infection in patients with acute febrile syndrome: Is a predictive model based solely on signs and symptoms useful?PloS one · 2022Article
- Observational Characterization of the Ecological and Environmental Features Associated with the Presence of Oropouche Virus and the Primary VectorTropical medicine and infectious disease · 2021Review
- Evolutionary Dynamics of Oropouche Virus in South America.Journal of virology · 2020Article
- Oropouche fever cases in Salvador, Bahia, Brazil, 2024.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
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Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
Abstract
Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published qRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian OROV lineage was identified from metagenomic sequencing data. We report the optimisation of an qRT-PCR assay for the Ecuadorian OROV lineage, which subsequently identified a further five cases in a cohort of 196 febrile patients. We isolated OROV via cell culture and developed an algorithmically-designed primer set for whole-genome amplification of the virus. Metagenomic sequencing of the patient samples provided OROV genome coverage ranging from 68-99%. The additional cases formed a single phylogenetic cluster together with the initial case. OROV should be considered as a differential diagnosis for Ecuadorian patients with febrile illness to avoid mis-diagnosis with other circulating pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.