ArticleJournal of experimental botany2020
Transposon-induced methylation of the RsMYB1 promoter disturbs anthocyanin accumulation in red-fleshed radish.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 71 citations in OpenAlex.
- Transposable elements and their epigenetic modifications mediate gene expression and subgenome dominance in Brassica carinata.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Understanding epigenetic regulation in nonmodel plants: transcriptomic responses to seed demethylation in leaves and roots of the annual herb Erodium cicutarium (Geraniaceae).G3 (Bethesda, Md.) · 2026Article
- Long non-coding RNA RsLNC2361 positively regulate anthocyanin accumulation in radish.BMC plant biology · 2026Article
- Unraveling the genetic and molecular mechanisms of anthocyanin biosynthesis and accumulation in maize kernels.Frontiers in plant science · 2026Review
- Salicylic acid exerts effects on regulating flower bud differentiation and nutrient contents in red radish.Frontiers in plant science · 2026Article
- The ApWRKY26/ApERF4-ApMYB2 module regulates anthocyanin accumulation for the seasonal leaf color transition inHorticulture research · 2026Article
- Identification of regulatory genes involved in anthocyanin accumulation in radish leaves using bulk segregant analysis.Scientific reports · 2025Article
- Fine-Mapping of a Red-Skinned Taproot Gene in Radish (Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification and Characterization of the Class III Peroxidase Gene Family in Radish (International journal of molecular sciences · 2025Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Identification of Candidate Genes for Green Rind Color in Watermelon.Plants (Basel, Switzerland) · 2025Article
- Epigenetic Regulation of Anthocyanin Biosynthesis inInternational journal of molecular sciences · 2024Article
- Sequence and epigenetic variations of R2R3-MYB transcription factors determine the diversity of taproot skin and flesh colors in different cultivated types of radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Transposons are important contributors to gene expression variability under selection in rice populations.eLife · 2023Article
- A 43 Bp-Deletion in theInternational journal of molecular sciences · 2023Article
- The combination of DNA methylation and positive regulation of anthocyanin biosynthesis by MYB and bHLH transcription factors contributes to the petal blotch formation in Xibei tree peony.Horticulture research · 2023Article
- Effect of structural variation in the promoter region ofFrontiers in plant science · 2023Article
- The long noncoding RNAFrontiers in plant science · 2023Article
- The Flavonoid Biosynthesis and Regulation inInternational journal of molecular sciences · 2022Review
- Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells.Genes · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Red-fleshed radish (Raphanus sativus L.) is a unique cultivar whose taproot is rich in anthocyanins beneficial to human health. However, the frequent occurrence of white-fleshed mutants affects the purity of commercially produced radish and the underlying mechanism has puzzled breeders for many years. In this study, we combined quantitative trait location by genome resequencing and transcriptome analyses to identify a candidate gene (RsMYB1) responsible for anthocyanin accumulation in red-fleshed radish. However, no sequence variation was found in the coding and regulatory regions of the RsMYB1 genes of red-fleshed (MTH01) and white-fleshed (JC01) lines, and a 7372 bp CACTA transposon in the RsMYB1 promoter region occurred in both lines. A subsequent analysis suggested that the white-fleshed mutant was the result of altered DNA methylation in the RsMYB1 promoter. This heritable epigenetic change was due to the hypermethylated CACTA transposon, which induced the spreading of DNA methylation to the promoter region of RsMYB1. Thus, RsMYB1 expression was considerably down-regulated, which inhibited anthocyanin biosynthesis in the white-fleshed mutant. An examination of transgenic radish calli and the results of a virus-induced gene silencing experiment confirmed that RsMYB1 is responsible for anthocyanin accumulation. Moreover, the mutant phenotype was partially eliminated by treatment with a demethylating agent. This study explains the molecular mechanism regulating the appearance of white-fleshed mutants of red-fleshed radish.
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