ArticleEmerging infectious diseases2020
Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi.
Article in Emerging infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Absence of human yaws and detection of Haemophilus ducreyi in skin ulcers of school children near wildlife protected areas in Tanzania.PLoS neglected tropical diseases · 2026Article
- External quality assessment for yaws elimination in low- and middle-income countries using plasmid-based proficiency test items.PLoS neglected tropical diseases · 2026Article
- Rapid visual detection of Treponema pallidum using the RPA-CRISPR/Cas12a system.Scientific reports · 2026Article
- Mapping the distribution of yaws and Haemophilus ducreyi in the western north region of Ghana.BMC infectious diseases · 2025Observational
- Detection ofApplied and environmental microbiology · 2025Article
- Pathogen-Specific Electrochemical Real-Time LAMP Detection Using Universal Solid-Phase Probes on Carbon Electrodes.ACS sensors · 2025Article
- Prospective evaluation of non-invasive saliva specimens for the diagnosis of syphilis and molecular surveillance ofJournal of clinical microbiology · 2024Article
- Aptamer-associated colorimetric reverse transcription loop-mediated isothermal amplification assay for detection of dengue virus.Microbiology spectrum · 2024Article
- Field-applicable simultaneous multiplex LAMP assay for screening HBV and HCV co-infection in a single tube.BMC infectious diseases · 2024Article
- Multiplexing LAMP Assays: A Methodological Review and Diagnostic Application.International journal of molecular sciences · 2024Review
- Article
- Establishment and application of a loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) method for detecting Clostridium piliforme.Veterinary medicine and science · 2024Article
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Development and application of LAMP assays for the detection of enteric adenoviruses in feces.Microbiology spectrum · 2022Article
- LAMP4yaws:BMJ open · 2022Article
- Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection ofTropical medicine and infectious disease · 2020Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Yaws, a neglected tropical disease caused by the bacterium Treponema pallidum subspecies pertenue, manifests as ulcerative skin lesions. Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to distinguish yaws from infections that cause similar skin lesions, primarily Haemophilus ducreyi. We developed a novel molecular test to simultaneously detect T. pallidum and H. ducreyi based on mediator displacement LAMP. We validated the T. pallidum and H. ducreyi LAMP (TPHD-LAMP) by testing 293 clinical samples from patients with yaws-like lesions. Compared with quantitative PCR, the TPHD-LAMP demonstrated high sensitivity and specificity for T. pallidum (84.7% sensitivity, 95.7% specificity) and H. ducreyi (91.6% sensitivity, 84.8% specificity). This novel assay provided rapid molecular confirmation of T. pallidum and H. ducreyi DNA and might be suitable for use at the point of care. TPHD-LAMP could support yaws eradication by improving access to molecular diagnostic tests at the district hospital level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.