ArticleArthritis & rheumatology (Hoboken, N.J.)2020
Identification of Cartilage Microbial DNA Signatures and Associations With Knee and Hip Osteoarthritis.
Article in Arthritis & rheumatology (Hoboken, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 3 syntheses or guidelines pooled it, 98 citations in OpenAlex.
- Association of low-grade inflammation caused by gut microbiota disturbances with osteoarthritis: A systematic review.Frontiers in veterinary science · 2022Pooled it
- Gut permeability and osteoarthritis, towards a mechanistic understanding of the pathogenesis: a systematic review.Annals of medicine · 2021Pooled it
- The relation between the gut microbiome and osteoarthritis: A systematic review of literature.PloS one · 2021Pooled it
- Complement Expression and Activation in Osteoarthritis Joint Compartments.Frontiers in immunology · 2020Trial
- What does the gut microbiome teach us about osteoarthritis development and progression?Best practice & research. Clinical rheumatology · 2026Review
- The gut-joint axis in osteoarthritis.Nature reviews. Rheumatology · 2026Review
- A Potential Central Hub of Histamine in the Microbiota-Gut-Joint Axis in Rheumatoid Arthritis: Mechanisms and Translational Implications.International journal of molecular sciences · 2026Review
- Analysis of gut microbiota characteristics in osteoarthritis patients.Frontiers in cellular and infection microbiology · 2026Article
- Deciphering osteoarthritis cartilage heterogeneity: gene expression-guided patient stratification and microbiome correlations.Frontiers in microbiology · 2026Article
- Hip Osteoarthritis and the Human Microbiome: Current Concepts, Biological Insights and Future Directions.Orthopedic reviews · 2026Article
- The brain-gut-joint axis in arthritis: crosstalk, treatment, and future perspectives.Journal of orthopaedic translation · 2026Review
- Bacterial extracellular vesicles in osteoarthritis: a new bridge of the gut-joint axis.Gut microbes · 2025Review
- Gut microbiome dysbiosis accelerates osteoarthritis progression by inducing IFP-SM inflammation in "double-hit" mice.Arthritis research & therapy · 2025Article
- Is Osteoarthritis a State of Joint Dysbiosis?Antibiotics (Basel, Switzerland) · 2025Review
- Effect of electroacupuncture combined with Tuina therapy on gut microbiota in patients with knee osteoarthritis.World journal of gastroenterology · 2025Article
- Article
- Gut and Joint Microbiomes: Implications in Osteoarthritis.Rheumatic diseases clinics of North America · 2025Review
- The role of the immune system in osteoarthritis: mechanisms, challenges and future directions.Nature reviews. Rheumatology · 2025Review
- Progress in multi-omics studies of osteoarthritis.Biomarker research · 2025Review
- Gut microbiota and osteoarthritis: epidemiology, mechanistic analysis, and new horizons for pharmacological interventions.Frontiers in cellular and infection microbiology · 2025Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveAlterations of the gut microbiota have been implicated in many forms of arthritis, but an examination of cartilage microbial patterns has not been performed. This study was undertaken to characterize the microbial DNA profile of articular cartilage and determine changes associated with osteoarthritis (OA).
methodsWe performed 16S ribosomal RNA gene deep sequencing on eroded and intact cartilage samples from knee OA patients (n = 21 eroded and 21 intact samples) and hip OA patients (n = 34 eroded and 33 intact samples) and cadaver controls (n = 10 knee samples and 10 hip samples). Microbial DNA diversity was assessed, groups were compared, and metagenomic profiles were reconstructed. Confirmation was performed in an independent cohort by clade-specific quantitative polymerase chain reaction. Findings in human cartilage were compared to those in cartilage from OA-susceptible C57BL/6 (B6) mice and OA-resistant MRL/MpJ (MRL) mice. Germ-free B6 mouse cartilage was analyzed as a methodologic control.
resultsAlpha diversity was reduced in human OA versus control samples (P < 0.0001), and in hip versus knee samples (P < 0.0001). Numerous clades were different in human OA versus control samples, and similar findings were noted in comparisons of murine B6 versus MRL mice. Hip samples were microbiologically distinct from knee samples. OA microbial DNA demonstrated increased gram-negative constituents (P = 0.02). Functional analysis demonstrated increases in lipopolysaccharide production (P = 9.9 × 10
conclusionOur study reveals a microbial DNA signature in human and mouse cartilage. Alterations in this signature, including increases in gram-negative constituents, occur during the development and progression of human OA. Furthermore, our findings indicate that strain-specific signatures exist within mouse cartilage that mirror human patterns. Further study of the establishment and potential pathogenic role of these DNA signatures is needed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.