ArticleMolecular microbiology2020
The effects of polydisperse crowders on the compaction of the Escherichia coli nucleoid.
Article in Molecular microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Crowding-induced collapse and adsorption of polymers with nonuniform bending stiffness.Soft matter · 2026Article
- Dual Role of Small Noncoding RNA and Hfq in Bacterial DNA Compaction: A New Perspective on Nucleoid Architecture.ACS omega · 2026Article
- Gravity as a Boundary Condition for the Evolution of Three-Dimensional Multicellularity.Life (Basel, Switzerland) · 2026Article
- Abundant positively-charged proteins underlie JCVI-Syn3A's expanded nucleoid and ribosome distribution.PLoS computational biology · 2026Article
- Review
- The role of active mRNA-ribosome dynamics and closing constriction in daughter chromosome separation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Crowding-induced collapse and adsorption of polymers with nonuniform bending stiffness.bioRxiv : the preprint server for biology · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- Insights on the effect of macromolecular crowding on transcription and its regulation.QRB discovery · 2025Article
- Antisense transcription is associated with expression of metal resistance determinants in Cupriavidus metallidurans CH34.Metallomics : integrated biometal science · 2024Article
- Differentiating the roles of proteins and polysomes in nucleoid size homeostasis in Escherichia coli.Biophysical journal · 2024Article
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Contribution of different macromolecules to the diffusion of a 40 nm particle in Escherichia coli.Biophysical journal · 2024Article
- Target search by an imported conjugative DNA element for a unique integration site along a bacterial chromosome during horizontal gene transfer.Nucleic acids research · 2023Article
- Article
- Loss of Mobile Genomic Islands in Metal-Resistant, Hydrogen-Oxidizing Cupriavidus metallidurans.Applied and environmental microbiology · 2022Article
- Steric interactions and out-of-equilibrium processes control the internal organization of bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Robust surface-to-mass coupling and turgor-dependent cell width determine bacterial dry-mass density.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Lab-on-a-chip based mechanical actuators and sensors for single-cell and organoid culture studies.Journal of applied physics · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
DNA binding proteins, supercoiling, macromolecular crowders, and transient DNA attachments to the cell membrane have all been implicated in the organization of the bacterial chromosome. However, it is unclear what role these factors play in compacting the bacterial DNA into a distinct organelle-like entity, the nucleoid. By analyzing the effects of osmotic shock and mechanical squeezing on Escherichia coli, we show that macromolecular crowders play a dominant role in the compaction of the DNA into the nucleoid. We find that a 30% increase in the crowder concentration from physiological levels leads to a three-fold decrease in the nucleoid's volume. The compaction is anisotropic, being higher along the long axes of the cell at low crowding levels. At higher crowding levels, the nucleoid becomes spherical, and its compressibility decreases significantly. Furthermore, we find that the compressibility of the nucleoid is not significantly affected by cell growth rates and by prior treatment with rifampicin. The latter results point out that in addition to poly ribosomes, soluble cytoplasmic proteins have a significant contribution in determining the size of the nucleoid. The contribution of poly ribosomes dominates at faster and soluble proteins at slower growth rates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.