ArticleBMC plant biology2020
Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.).
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Metabolic engineering of tobacco plants using Carica papaya NAC transcription factors: a biotechnology tool to improve plant metabolite production.Biotechnology letters · 2026Article
- Nicotine biosynthesis is completed by cryptic activating glucosylation.Nature communications · 2026Article
- Genome-wide identification, expression analysis of the JmjC gene family in tobacco and functional characterization of NtJMJ19 role in nicotine biosynthesis.BMC plant biology · 2026Article
- Overexpression of NtGSTU10 gene enhances nicotine content in hybrids by promoting the synthesis and transport metabolism of nicotine.BMC biotechnology · 2025Article
- Metabolomic and transcriptomic analyses reveal differences in fatty acids in tobacco leaves across cultivars and developmental stages.BMC plant biology · 2025Article
- Multiomics provides insights into dynamic changes of aromatic profile during flue-curing process in tobacco (Nicotiana tabacum L.) leaves.BMC plant biology · 2025Article
- Microstructure observation and flavor substances excavation of Yunyan 87 tobacco leaves with different oil contents.Frontiers in plant science · 2025Article
- Integration of QTL mapping and GWAS reveals the complicated genetic architecture of chemical composition traits in tobacco leaves.Frontiers in plant science · 2025Article
- Integrated analysis of physiological and metabolic data uncovers essential dynamic mechanisms involved in the maturation of cigar tobacco leaves.Scientific reports · 2024Article
- Integrative proteome and metabolome unveil the central role of IAA alteration in axillary bud development following topping in tobacco.Scientific reports · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Proteomic and metabolomic insights into the impact of topping treatment on cigar tobacco.Frontiers in plant science · 2024Article
- Topping and NPK fertilization alter seed germination, plant growth and active components ofFrontiers in plant science · 2024Article
- Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).BMC genomics · 2023Article
- Article
- Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance inFrontiers in plant science · 2023Article
- Topping and grafting affect the alkaloid content and gene expression patterns of tobacco (Plant direct · 2023Article
- Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties.BMC plant biology · 2022Article
- Article
- The tomato cytochrome P450 CYP712G1 catalyses the double oxidation of orobanchol en route to the rhizosphere signalling strigolactone, solanacol.The New phytologist · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundNicotiana tabacum is an important economic crop. Topping, a common agricultural practice employed with flue-cured tobacco, is designed to increase leaf nicotine contents by increasing nicotine biosynthesis in roots. Many genes are found to be differentially expressed in response to topping, particularly genes involved in nicotine biosynthesis, but comprehensive analyses of early transcriptional responses induced by topping are not yet available. To develop a detailed understanding of the mechanisms regulating nicotine biosynthesis after topping, we have sequenced the transcriptomes of Nicotiana tabacum roots at seven time points following topping.
resultsDifferential expression analysis revealed that 4830 genes responded to topping across all time points. Amongst these, nine gene families involved in nicotine biosynthesis and two gene families involved in nicotine transport showed significant changes during the immediate 24 h period following topping. No obvious preference to the parental species was detected in the differentially expressed genes (DEGs). Significant changes in transcript levels of nine genes involved in nicotine biosynthesis and phytohormone signal transduction were validated by qRT-PCR assays. 549 genes encoding transcription factors (TFs), found to exhibit significant changes in gene expression after topping, formed 15 clusters based on similarities of their transcript level time-course profiles. 336 DEGs involved in phytohormone signal transduction, including genes functionally related to the phytohormones jasmonic acid, abscisic acid, auxin, ethylene, and gibberellin, were identified at the earliest time point after topping.
conclusionsOur research provides the first detailed analysis of the early transcriptional responses to topping in N. tabacum, and identifies excellent candidates for further detailed studies concerning the regulation of nicotine biosynthesis in tobacco roots.
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