ArticleMicrobial biotechnology2020
Controlled replication of 'Candidatus Liberibacter asiaticus' DNA in citrus leaf discs.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- A Citrus medica stem cutting-based platform for rapid screening of therapeutic compounds against Candidatus Liberibacter asiaticus.Scientific reports · 2026Article
- Galleria mellonella possesses the essential nutritional needs to host the fastidious Huanglongbing bacterial pathogen 'Candidatus Liberibacter asiaticus'.Communications biology · 2025Article
- Rootstock and Crop Load Effects on 'Honeycrisp' Photosynthetic Performance and Carbohydrate Accumulation.Plants (Basel, Switzerland) · 2023Article
- A synthetic 'essentialome' for axenic culturing of 'Candidatus Liberibacter asiaticus'.BMC research notes · 2022Article
- Molecular signatures between citrus and Candidatus Liberibacter asiaticus.PLoS pathogens · 2021Review
- Melatonin Is Involved in Citrus Response to the Pathogen Huanglongbing via Modulation of Phytohormonal Biosynthesis.Plant physiology · 2020Article
- The Role of the Xylem in Oxytetracycline Translocation within Citrus Trees.Antibiotics (Basel, Switzerland) · 2020Article
- Revisiting the Complex Pathosystem of Huanglongbing: Deciphering the Role of Citrus Metabolites in Symptom Development.Metabolites · 2020Review
- Controlled replication of 'Candidatus Liberibacter asiaticus' DNA in citrus leaf discs.Microbial biotechnology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
'Candidatus Liberibacter asiaticus' is a fastidious bacterium and a putative agent of citrus greening disease (a.k.a., huanglongbing, HLB), a significant agricultural disease that affects citrus fruit quality and tree health. In citrus, 'Ca. L. asiaticus' is phloem limited. Lack of culture tools to study 'Ca. L. asiaticus' complicates analysis of this important organism. To improve understanding of 'Ca. L. asiaticus'-host interactions including parameters that affect 'Ca. L. asiaticus' replication, methods suitable for screening pathogen responses to physicochemical and nutritional variables are needed. We describe a leaf disc-based culture assay that allows highly selective measurement of changes in 'Ca. L. asiaticus' DNA within plant tissue incubated under specific physicochemical and nutritional conditions. qPCR analysis targeting the hypothetical gene CD16-00155 (strain A4) allowed selective quantification of 'Ca. L. asiaticus' DNA content within infected tissue. 'Ca. L. asiaticus' DNA replication was observed in response to glucose exclusively under microaerobic conditions, and the antibiotic amikacin further enhanced 'Ca. L. asiaticus' DNA replication. Metabolite profiling revealed a moderate impact of 'Ca. L. asiaticus' on the ability of leaf tissue to metabolize and respond to glucose.
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