ArticleReproduction (Cambridge, England)2020
Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.
Article in Reproduction (Cambridge, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- A Testis-Specific Aralkylamine N-Acetyltransferase Regulates Dimorphic Sperm Function and Male Fertility in Moths.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Intraflagellar transport-associated CCDC92 is required for spermiogenesis and male fertility in mice.Journal of molecular cell biology · 2025Article
- Male infertility.Systems biology in reproductive medicine · 2025Review
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Preparation of Testicular Cells for Immunofluorescence Analysis of Manchette in Elongating Spermatids.Bio-protocol · 2025Article
- Function of manchette and intra-manchette transport in spermatogenesis and male fertility.Cell communication and signaling : CCS · 2025Review
- Immunofluorescence Staining of the Manchette and Developing Sperm Flagella in Mouse.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Intraflagellar Transport (IFT) and Sperm Formation.Advances in experimental medicine and biology · 2025Review
- Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations.Human molecular genetics · 2023Article
- Article
- IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect.EMBO molecular medicine · 2022Article
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters.Reproduction & fertility · 2021Article
- AXDND1, a novel testis-enriched gene, is required for spermiogenesis and male fertility.Cell death discovery · 2021Article
- Sperm Methylome Profiling Can Discern Fertility Levels in the Porcine Biomedical Model.International journal of molecular sciences · 2021Article
- Some thoughts about intraflagellar transport in reproduction.Molecular reproduction and development · 2021Article
- Genetic Defects inFrontiers in cell and developmental biology · 2021Article
- Sperm Differentiation: The Role of Trafficking of Proteins.International journal of molecular sciences · 2020Review
- Molecular basis of the morphogenesis of sperm head and tail in mice.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Intraflagellar transport (IFT) is a conserved mechanism essential for the assembly and maintenance of most eukaryotic cilia and flagella. IFT172 is a component of the IFT complex. Global disruption of mouse Ift172 gene caused typical phenotypes of ciliopathy. Mouse Ift172 gene appears to translate two major proteins; the full-length protein is highly expressed in the tissues enriched in cilia and the smaller 130 kDa one is only abundant in the testis. In male germ cells, IFT172 is highly expressed in the manchette of elongating spermatids. A germ cell-specific Ift172 mutant mice were generated, and the mutant mice did not show gross abnormalities. There was no difference in testis/body weight between the control and mutant mice, but more than half of the adult homozygous mutant males were infertile and associated with abnormally developed germ cells in the spermiogenesis phase. The cauda epididymides in mutant mice contained less developed sperm that showed significantly reduced motility, and these sperm had multiple defects in ultrastructure and bent tails. In the mutant mice, testicular expression levels of some IFT components, including IFT20, IFT27, IFT74, IFT81 and IFT140, and a central apparatus protein SPAG16L were not changed. However, expression levels of ODF2, a component of the outer dense fiber, and AKAP4, a component of fibrous sheath, and two IFT components IFT25 and IFT57 were dramatically reduced. Our findings demonstrate that IFT172 is essential for normal male fertility and spermiogenesis in mice, probably by modulating specific IFT proteins and transporting/assembling unique accessory structural proteins into spermatozoa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.