Evidence map›Paper›PMID 31958155›Full record

ArticleDrug development research2020

Discovery of a novel fluorescent chemical probe suitable for evaluation of neuropilin-1 binding of small molecules.

Daniel Conole, Yi-Tai Chou, Anastasia Patsiarika, Valery Nwabo, Eleni Dimitriou, Christelle Soudy, Filipa Mota, Snezana Djordjevic, David L Selwood

Erratum issuedOpen access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Drug development research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. SARS-CoV-2 cell entry beyond the ACE2 receptor.Molecular biology reports · 2022
    Pooled it
  2. Article
  3. Neuropilins: C-end rule peptides and their association with nociception and COVID-19.Computational and structural biotechnology journal · 2021
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Daniel ConoleWolfson Institute for Biomedical Research, University College London, London, UK.
Yi-Tai ChouWolfson Institute for Biomedical Research, University College London, London, UK.
Anastasia PatsiarikaWolfson Institute for Biomedical Research, University College London, London, UK.
Valery NwaboWolfson Institute for Biomedical Research, University College London, London, UK.
Eleni DimitriouWolfson Institute for Biomedical Research, University College London, London, UK.
Christelle SoudyWolfson Institute for Biomedical Research, University College London, London, UK.
Filipa MotaWolfson Institute for Biomedical Research, University College London, London, UK.
Snezana DjordjevicInstitute of Structural and Molecular Biology, University College London, London, UK.
David L SelwoodWolfson Institute for Biomedical Research, University College London, London, UK.ORCID 0000-0002-6817-5064
Wolfson Foundation · GBInstitute of Structural and Molecular Biology · GB

Funding

British Heart Foundation PG/10/52/28448
6 · The paper itself

Abstract

Neuropilin-1 (NRP1) is emerging as an important molecule in immune signaling where it has been shown to modulate the actions of TGF-β1 in macrophages and regulatory T cells. The development of cost-effective and reliable assays for NRP1 binding is therefore important. We synthesized three new NRP1 small molecule fluorophores and examined their performance as fluorescent polarization probes. One molecule DS108 exhibited favorable binding and fluorescent characteristics and allowed us to establish a simple assay suitable for medium to high throughput screening of small molecules.

Indexed as

Fluorescent DyesHigh-Throughput Screening AssaysNeuropilin-1Signal TransductionTransforming Growth Factor beta1Fluorescent DyesNeuropilin-1Transforming Growth Factor beta1fluorescence polarizationneuropilin-1surface plasmon resonance

Identifiers

PMID31958155
PMCPMC7317715
OpenAlexW3002619270

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.