ArticleAdvanced healthcare materials2020
Relaxation of Extracellular Matrix Forces Directs Crypt Formation and Architecture in Intestinal Organoids.
Article in Advanced healthcare materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 101 citations in OpenAlex.
- Photoresponsive Granular Hydrogels Enable Spatiotemporal Control of Matrix Mechanics and MSC Behavior.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Human gastric extracellular matrix hydrogels maintain organoid growth and reduce gene expression associated with stemness and inflammation.Biomedical materials (Bristol, England) · 2026Article
- Synthetic PEG-4MAL Hydrogels Support Patient-Derived Human Intestinal Enteroid Culture.Cell biomaterials · 2026Article
- Microscale Mechanical Cues in Hydrogels: Engineering Strategies to Modulate Cell Fates in Three Dimensions.Cell biomaterials · 2026Article
- Lipoengineering of Biomolecular Condensates Controls Material Properties and Multiphase Hierarchy to Guide Organoid Morphogenesis.bioRxiv : the preprint server for biology · 2026Article
- The Synthetic Extracellular Matrix as a Maestro of the In Vitro Stem Cell Niche: Orchestrating Fate and Function.Biomedicines · 2026Review
- Dynamic hydrogel mechanics in organoid engineering: From matrix design to translational paradigms.Bioactive materials · 2026Review
- AnBiofabrication · 2025Article
- Fully Synthetic Hydrogels Promote Robust Crypt Formation in Intestinal Organoids.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Engineered basement membrane mimetic hydrogels to study mammary epithelial morphogenesis and invasion.Science advances · 2025Article
- Stem Cells and Organoids: A Paradigm Shift in Preclinical Models Toward Personalized Medicine.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Advancements and Challenges in Modeling Mechanobiology in Intestinal Host-Microbiota Interaction.ACS applied materials & interfaces · 2025Review
- Mechanically and Chemically Defined PEG Hydrogels Improve Reproducibility in Human Cardioid Development.Advanced healthcare materials · 2025Article
- Engineered epithelial curvature controls Paneth cell localization in intestinal organoids.Cell biomaterials · 2025Article
- Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review.Molecular medicine (Cambridge, Mass.) · 2025Review
- Visible light-responsive hydrogels for cellular dynamics and spatiotemporal viscoelastic regulation.Nature communications · 2025Article
- Light-based fabrication and 4D customization of hydrogel biomaterials.Nature reviews bioengineering · 2025Article
- Synthetic photoresponsive hydrogels enableAdvanced functional materials · 2025Article
- Photoresponsive Chemistries for User-Directed Hydrogel Network Modulation to Investigate Cell-Matrix Interactions.Accounts of chemical research · 2025Review
- Organoid scaffold materials: research and application.Frontiers in bioengineering and biotechnology · 2025Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Intestinal organoid protocols rely on the use of extracellular scaffolds, typically Matrigel, and upon switching from growth to differentiation promoting media, a symmetry breaking event takes place. During this stage, the first bud like structures analogous to crypts protrude from the central body and differentiation ensues. While organoids provide unparalleled architectural and functional complexity, this sophistication is also responsible for the high variability and lack of reproducibility of uniform crypt-villus structures. If function follows form in organoids, such structural variability carries potential limitations for translational applications (e.g., drug screening). Consequently, there is interest in developing synthetic biomaterials to direct organoid growth and differentiation. It has been hypothesized that synthetic scaffold softening is necessary for crypt development, and these mechanical requirements raise the question, what compressive forces and subsequent relaxation are necessary for organoid maturation? To that end, allyl sulfide hydrogels are employed as a synthetic extracellular matrix mimic, but with photocleavable bonds that temporally regulate the material's bulk modulus. By varying the extent of matrix softening, it is demonstrated that crypt formation, size, and number per colony are functions of matrix softening. An understanding of the mechanical dependence of crypt architecture is necessary to instruct homogenous, reproducible organoids for clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.