ArticleJournal, genetic engineering & biotechnology2020
In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant.
Article in Journal, genetic engineering & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Microbial Diversity Analysis of Soil in the Rhizosphere ofMicroorganisms · 2025Article
- Efficient In Vitro Sterilization and Propagation from Stem Segment Explants ofPlants (Basel, Switzerland) · 2022Article
- High-frequency adventitious shoot organogenesis fromBiotechnologia · 2022Article
- Article
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Abstract
backgroundSecuridaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants.
resultsAmong different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization.
conclusionsThis micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement.
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