ArticleBiological procedures online2020
Analytical Validation of Telomere Analysis Technology® for the High-Throughput Analysis of Multiple Telomere-Associated Variables.
Article in Biological procedures online, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Association between telomere length, frailty and death in older adults.GeroScience · 2021Pooled it
- Trial
- Senescence-associated loss of intestinal α1,2-fucose disrupts a modifiable host-microbiome homeostasis axis in people with HIV.bioRxiv : the preprint server for biology · 2026Article
- Telomere Length Abnormality: Investigating Approaches and Correlations with Cancer, Bone Marrow Failure and Hematological Malignancies.Biomedicines · 2025Review
- Methods for telomere length measurement: an update on current technologies and emerging approaches.Frontiers in molecular biosciences · 2025Review
- Effect of an 18-Month Meditation Training on Telomeres in Older Adults: A Secondary Analysis of the Age-Well Randomized Controlled Trial.Biological psychiatry global open science · 2025Article
- Article
- Distinct intestinal microbial signatures linked to accelerated systemic and intestinal biological aging.Microbiome · 2024Article
- In Vitro Assessment of Gold Nanoparticles on Telomerase Activity and Telomere Length in Human Fibroblasts.International journal of molecular sciences · 2023Article
- Preclinical assessment of antigen-specific chimeric antigen receptor regulatory T cells for use in solid organ transplantation.Gene therapy · 2023Article
- Characterization of human induced pluripotent stems cells: Current approaches, challenges, and future solutions.Biotechnology reports (Amsterdam, Netherlands) · 2023Review
- High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform.Nature communications · 2023Article
- In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient.Molecules (Basel, Switzerland) · 2022Article
- Molecular insight of dyskeratosis congenita: Defects in telomere length homeostasis.Journal of clinical and translational research · 2022Review
- Engineering osteoarthritic cartilage model through differentiating senescent human mesenchymal stem cells for testing disease-modifying drugs.Science China. Life sciences · 2022Article
- Long-Term Exposure to Nanosized TiOInternational journal of molecular sciences · 2021Article
- Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.PloS one · 2021Article
- Mathematical Connection between Short Telomere Induced Senescence Calculation and Mortality Rate Data.International journal of molecular sciences · 2020Article
- Telomere Length as a Marker of Biological Age: State-of-the-Art, Open Issues, and Future Perspectives.Frontiers in genetics · 2020Review
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA large number of studies have suggested a correlation between the status of telomeres and disease risk. High-throughput quantitative fluorescence in situ hybridization (HT Q-FISH) is a highly accurate telomere measurement technique that can be applied to the study of large cell populations. Here we describe the analytical performance testing and validation of Telomere Analysis Technology (TAT®), a laboratory-developed HT Q-FISH-based methodology that includes HT imaging and software workflows that provide a highly detailed view of telomere populations.
methodsTAT was developed for the analysis of telomeres in peripheral blood mononuclear cells (PBMCs). TAT was compared with Terminal Restriction Fragment (TRF) length analysis, and tested for accuracy, precision, limits of detection (LOD) and specificity, reportable range and reference range.
resultsUsing 6 different lymphocyte cell lines, we found a high correlation between TAT and TRF for telomere length (R
conclusionsAnalytical validation of TAT shows that is a robust and reliable technique for the characterization of a detailed telomere profile in large cell populations. The combination of high-throughput imaging and software workflows allows for the collection of a large number of telomere-associated variables from each sample, which can then be used in epidemiological and clinical studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.