ArticleReproductive medicine and biology2020
Cell-free DNA content in follicular fluid: A marker for the developmental ability of porcine oocytes.
Article in Reproductive medicine and biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- The mitochondrial DNA copy number and ovary-related reproductive disorders: A bidirectional two-sample Mendelian randomization study.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2025Article
- Biochemical profiling of the follicular environment to predict oocyte competence in cattle.PloS one · 2024Article
- Article
- Elevated cell-free mitochondria DNA level of patients with premature ovarian insufficiency.BMC pregnancy and childbirth · 2023Article
- Resveratrol enhanced mitochondrial recovery from cryopreservation-induced damages in oocytes and embryos.Reproductive medicine and biology · 2021Review
- Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos.The Journal of reproduction and development · 2021Article
- Cell-free DNA discoveries in human reproductive medicine: providing a new tool for biomarker and genetic assays in ART.Journal of assisted reproduction and genetics · 2021Review
- Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos.The Journal of reproduction and development · 2020Article
- Abundance of cell-free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts.Reproductive medicine and biology · 2020Article
- Cell-free DNA content in follicular fluid: A marker for the developmental ability of porcine oocytes.Reproductive medicine and biology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe present study examined the relationships among the amount of cell-free-DNA (cfDNA) in porcine follicular fluid (FF), the developmental ability of enclosed oocytes, and characteristics of granulosa cells and examined the effect of cfDNA content in maturation medium on the developmental ability of the oocytes.
methodsOocytes and FF were collected from individual gilts, and the gilts were rated based on the ability of their oocytes to develop to the blastocyst stage and the amount of cfDNA in the FF. The copy numbers of mitochondrial DNA (Mt-DNA) and nuclear DNA (N-DNA) were measured by real-time PCR and the DNA sequence. FF or cfDNA was added to the maturation medium, and the developmental ability of the oocytes was examined.
resultsThe amount of cfDNA was associated with apoptosis of the granulosa cells, and high-cfDNA content in FF was associated with low developmental ability of oocytes. Supplementation of the maturation medium with FF containing high cf-Mt-DNA or with DNA extracted from the FF did not affect oocyte developmental competence.
conclusionsCell-free DNA content in FF is a marker for oocyte competence, but cfDNA in the oocyte maturation environment did not affect oocyte developmental ability.
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