ArticleMolecular microbiology2020
The Lon-2 protease of Borrelia burgdorferi is critical for infection in the mammalian host.
Article in Molecular microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Virulence Regulation inMicroorganisms · 2025Review
- Borrelia burgdorferi serine protease HtrA is a pleiotropic regulator of stress response, motility, flagellar hemostasis, and infectivity.Communications biology · 2025Article
- Analysis of the BadR regulon in Borrelia burgdorferi.BMC microbiology · 2025Article
- Article
- Transcriptome analysis ofFrontiers in microbiology · 2025Article
- Analysis of bb0556 Expression and Its Role During Borrelia burgdorferi Mammalian Infection.Molecular microbiology · 2024Article
- Assessment of the hypothetical protein BB0616 in the murine infection ofInfection and immunity · 2024Article
- A chemosensory-like histidine kinase is dispensable for chemotaxis in vitro but regulates the virulence of Borrelia burgdorferi through modulating the stability of RpoS.PLoS pathogens · 2023Article
- Role of the Hypothetical Protein BB0563 during Borrelia burgdorferi Infection in Animals.Infection and immunity · 2023Article
- BB0761, a MepM homolog, contributes to Borrelia burgdorferi cell division and mammalian infectivity.Molecular microbiology · 2022Article
- Endogenous and Borrowed Proteolytic Activity in theMicrobiology and molecular biology reviews : MMBR · 2021Review
- Lyme Disease Pathogenesis.Current issues in molecular biology · 2021Review
- DksA plays an essential role in regulating the virulence of Borrelia burgdorferi.Molecular microbiology · 2020Article
- The Lon-1 Protease Is Required by Borrelia burgdorferi To Infect the Mammalian Host.Infection and immunity · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Borrelia burgdorferi encodes a functional homolog of canonical Lon protease termed Lon-2. To date, the contribution of Lon-2 to B. burgdorferi fitness and infection remains unexplored. Herein, we showed that expression of lon-2 was highly induced during animal infection, suggesting that Lon-2 is important for B. burgdorferi infection. We further generated a lon-2 deletion mutant. Compared with that of wild-type (WT) strain, the infectivity of the mutant was severely attenuated in a murine infection model. Although no growth defect was observed for the mutant in normal BSK-II medium, resistance of the lon-2 mutant to osmotic stress was markedly reduced. In addition, when exposed to tert-Butyl hydroperoxide, survival of the lon-2 mutant was impaired. In addition, we found that the protein levels of RpoS and RpoS-dependent OspC were decreased in the mutant. All these phenotypes were restored to WT or near-WT levels when lon-2 mutation was complemented in cis. Taken together, these results demonstrate that Lon-2 is critical for B. burgdorferi to establish infection and to cope with environmental stresses. This study provides a foundation for further uncovering the direct link between the dual roles of Lon-2 in protein quality control and bacterial pathogenesis.
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