ArticleAMB Express2020
Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions.
Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Enzymatic Deamidation of Plant Proteins by Protein-Glutamine Glutaminases: Effects on Solubility, Foaming and Emulsion Properties.Foods (Basel, Switzerland) · 2026Article
- Secretory Production of Mature Protein-Glutaminase from Bacteroides helcogenes in Bacillus subtilis.Applied biochemistry and biotechnology · 2026Article
- Directed screening and spatial coupling of farnesyl diphosphate synthase for enhancing menaquinone-7 production in Bacillus subtilis.Microbial cell factories · 2026Article
- Toward food-grade production of the Bacteroides helcogenes protein-glutamine glutaminase with an optimized Bacillus subtilis strain.Applied microbiology and biotechnology · 2026Article
- Recombinant production of acidophilic L-arabinose isomerase from Lentilactobacillus parakefiri in Bacillus subtilis.Microbial cell factories · 2025Article
- Importance of the 5' untranslated region for recombinant enzyme production in isolated Bacillus subtilis 007.AMB Express · 2025Article
- Secretion of the cytoplasmic and high molecular weight β-galactosidase of Paenibacillus wynnii with Bacillus subtilis.Microbial cell factories · 2024Article
- Optimum Fermentation Conditions for Bovine Lactoferricin-Lactoferrampin-EncodingFoods (Basel, Switzerland) · 2023Article
- Pullulanase: unleashing the power of enzyme with a promising future in the food industry.Frontiers in bioengineering and biotechnology · 2023Review
- Properties of a neutral, thermally stable and surfactant-tolerant pullulanase from worker termite gut-dwellingHeliyon · 2022Article
- A 4-α-Glucanotransferase from Thermus thermophilus HB8: Secretory Expression and Characterization.Current microbiology · 2022Article
- An overview and future prospects of recombinant protein production in Bacillus subtilis.Applied microbiology and biotechnology · 2021Review
- Biotechnology and bioengineering of pullulanase: state of the art and perspectives.World journal of microbiology & biotechnology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Pullulanase is an important starch-debranching enzyme mostly used in starch processing-related food industries. However, the levels of pullulanase produced from recombinant Bacillus subtilis, a Generally Recognized as Safe (GRAS) host, are generally limited. To enhance the activity of pullulanase, batch fermentation and fed-batch fermentation were systematically investigated. The overall purpose is to improve the fermentation yield by optimizing the feeding strategy in the fermentation process, thereby increasing the enzyme activity of pullulanase. Therefore, in this study, the feeding methods, the feeding ingredients, the feeding concentration, and pH values were studied in detail. The optimized fermentation conditions for pullulanase production from recombinant B. subtilis were determined as following: inoculum volume 7%, pH 6.5, the dissolved oxygen level 30%, and constant-rate feeding of 100 mL glucose solution (400 g L
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