Evidence map›Paper›PMID 31955298›Full record

ArticleOdontology2020

Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth.

Yohei Nakayama, Eiko Inoue, Ayako Kato, Yasunobu Iwai, Mizuho Takai-Yamazaki, Yuto Tsuruya, Arisa Yamaguchi, Keisuke Noda, Takato Nomoto, Bernhard Ganss and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Odontology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Yohei NakayamaDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan. nakayama.youhei@nihon-u.ac.jp.ORCID http://orcid.org/0000-0002-0823-7374
Eiko InoueDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Ayako KatoDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Yasunobu IwaiDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Mizuho Takai-YamazakiDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Yuto TsuruyaDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Arisa YamaguchiDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Keisuke NodaDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan.
Takato NomotoResearch Institute of Oral Science, Nihon University School of Dentistry At Matsudo, Matsudo, Japan.
Bernhard GanssMatrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Yorimasa OgataDepartment of Periodontology, Nihon University School of Dentistry At Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba, 271-8587, Japan. ogata.yorimasa@nihon-u.ac.jp.
Nihon University · JPUniversity of Toronto · CA

Funding

Discovery Grant RGPIN 2019-07070the Japan Society for the Promotion of Science KAKENHI Grants, Grant-in-Aid for Scientific Research (C) 17K11994the Japan Society for the Promotion of Science KAKENHI Grants, Grant-in-Aid for Scientific Research (C) 18K09582the Natural Sciences and Engineering Research Council (NSERC) and an Operating Grant MOP 119310
6 · The paper itself

Abstract

Follicular dendritic cell-secreted protein (FDC-SP) is secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium (JE). Its expression could be controlled during inflammatory process of gingiva; however, responsible mechanism for gingival overgrowth and involvement of FDC-SP in clinical condition is still unclear. We hypothesized that JE-specific genes are associated with the initiation of drug-induced gingival enlargement (DIGE) called gingival overgrowth, and investigated the changes of JE-specific gene's expression and their localization in overgrown gingiva from the patients. Immunohistochemical analysis revealed that the FDC-SP localization was spread in overgrown gingival tissues. FDC-SP mRNA levels in GE1 and Ca9-22 cells were increased by time-dependent nifedipine treatments, similar to other JE-specific genes, such as Amelotin (Amtn) and Lamininβ3 subunit (Lamβ3), whereas type 4 collagen (Col4) mRNA levels were decreased. Immunocytochemical analysis showed that FDC-SP, AMTN, and Lamβ3 protein levels were increased in GE1 and Ca9-22 cells. Transient transfection analyses were performed using luciferase constructs including various lengths of human FDC-SP gene promoter, nifedipine increased luciferase activities of -345 and -948FDC-SP constructs. These results raise the possibility that the nifedipine-induced FDC-SP may be related to the mechanism responsible for gingival overgrowth does not occur at edentulous jaw ridges.

Indexed as

Dendritic Cells, FollicularGingival OvergrowthEpithelial AttachmentGingivaHumansNifedipineNifedipineAMTNFDC-SPGingival overgrowthJunctional epitheliumNifedipine

Identifiers

PMID31955298
OpenAlexW3000571580

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.