Evidence map›Paper›PMID 31954402›Full record

ArticleJournal of biomedical science2020

Differential expression of microRNA, miR-150 and enhancer of zeste homolog 2 (EZH2) in peripheral blood cells as early prognostic markers of severe forms of dengue.

Harsha Hapugaswatta, Pubudu Amarasena, Ranjan Premaratna, Kapila N Seneviratne, Nimanthi Jayathilaka

Open access · diamondAbstract read
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In one paragraph

Article in Journal of biomedical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 2 pooled it
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 34 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Cross Talk between MicroRNAs and Dengue Virus.The American journal of tropical medicine and hygiene · 2024
    Review
  9. Article
  10. Review
  11. Article
  12. The Role of the ATP-Binding Cassette A1 (ABCA1) in Human Disease.International journal of molecular sciences · 2021
    Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Regulation of Host Innate Immunity by Non-Coding RNAs During Dengue Virus Infection.Frontiers in cellular and infection microbiology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Harsha HapugaswattaDepartment of Chemistry, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka.
Pubudu AmarasenaNorth Colombo Teaching Hospital, Ragama, Sri Lanka.
Ranjan PremaratnaNorth Colombo Teaching Hospital, Ragama, Sri Lanka.
Kapila N SeneviratneDepartment of Chemistry, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka.
Nimanthi JayathilakaDepartment of Chemistry, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka. njayathi@kln.ac.lk.ORCID http://orcid.org/0000-0001-8741-3075
University of Kelaniya · LKColombo North Teaching Hospital · LK

Funding

National Science Foundation RG/2015/BT/02Organization for Women in Science for the Developing World 4500384850University of Kelaniya RP/03/SR/02/06/02/2016
6 · The paper itself

Abstract

backgroundDengue presents a wide clinical spectrum. Most patients recover following a self-limiting non-severe clinical course. A small proportion of patients progress to severe disease, mostly characterized by plasma leakage with or without hemorrhage. Early symptoms of severe dengue (SD) are similar to those of non-severe dengue fever (DF). Severe symptoms manifest after 3-5 days of fever, which can be life threatening due to lack of proper medications and inability to distinguish severe cases during the early stages. Early prediction of SD in patients with no warning signs who may later develop severe infection is very important for proper disease management to alleviate related complications and mortality. microRNA are small non-coding RNA molecules that regulate post-transcriptional gene expression. Due to the remarkable stability and the role of microRNA in gene expression, altered expression of microRNA was evaluated to explore clinically relevant prognostic markers of severe dengue.

methodsThe relative expression of microRNA hsa-let-7e (let-7e), hsa-miR-30b-5p (miR-30b), hsa-miR-30e-3p (miR-30e), hsa-miR-33a (miR-33a), and hsa-miR-150-5p (miR-150) and several putative target genes in peripheral blood cells (PBC) collected from 20 DF and 20 SD positive patients within 4 days from fever onset was evaluated by quantitative reverse transcription PCR (qRT-PCR).

resultsmiR-150 showed significant (P < 0.01) up regulation in PBC of SD patients compared to DF patients during the acute phase of infection. Expression of enhancer of zeste homolog 2 (EZH2) was significantly (P < 0.01) down regulated indicating that genes involved in epigenetic regulation are also differentially expressed in SD patients during the early stage of infection.

conclusionsDifferential expression of microRNA miR-150 and the putative target gene EZH2 may serve as reliable biomarkers of disease severity during early stages of dengue infection.

Indexed as

Gene ExpressionGenetic MarkersAdultBlood CellsEnhancer of Zeste Homolog 2 ProteinFemaleHumansMaleMicroRNAsMiddle AgedPrognosisSevere DengueYoung AdultEnhancer of Zeste Homolog 2 ProteinGenetic MarkersMicroRNAsMIR150, humanAcute dengue biomarkersDenguemicroRNASevere dengue

Identifiers

PMID31954402
PMCPMC6969970
OpenAlexW3008380905

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.