Evidence map›Paper›PMID 31953639›Full record

ArticleStem cell reviews and reports2020

Isolation Methods for Human CD34 Subsets Using Fluorescent and Magnetic Activated Cell Sorting: an In Vivo Comparative Study.

Himi Tripathi, Hsuan Peng, Renee Donahue, Lakshman Chelvarajan, Anuhya Gottipati, Bryana Levitan, Ahmed Al-Darraji, Erhe Gao, Ahmed Abdel-Latif, Bradley J Berron

Open access · greenAbstract readComparative Study
In one paragraph

Article in Stem cell reviews and reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
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  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Isolation and Culture of Non-adherent Cells for Cell Reprogramming.Journal of stem cells & regenerative medicine · 2022
    Article
  10. Review
  11. Cell Sources for Human In vitro Bone Models.Current osteoporosis reports · 2021
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Himi TripathiGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.
Hsuan PengGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.
Renee DonahueGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.
Lakshman ChelvarajanGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.
Anuhya GottipatiDepartment of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA.
Bryana LevitanGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.
Ahmed Al-DarrajiGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.
Erhe GaoThe Center for Translational Medicine, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Ahmed Abdel-LatifGill Heart and Vascular Institute and Division of Cardiovascular Medicine, University of Kentucky and the Lexington VA Medical Center, Lexington, KY, USA.ORCID 0000-0002-8178-7102
Bradley J BerronDepartment of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA. brad.berron@uky.edu.
University of Kentucky · USTemple University · US

Funding

The role of IKKß in linking obesity to adipose tissue inflammation and adipogeneP20GM103527 · NIGMS · UNIVERSITY OF KENTUCKY · PI CASSIS, LISA A · 2012 to 2017
$13.7M
Pilot Projects ProgramP30GM127211 · NIGMS · UNIVERSITY OF KENTUCKY · PI CASSIS, LISA A · 2018 to 2022
$5.7M
Specific Lysis for Large-Volume, High-Purity Endothelial Progenitor Cell IsolatesR01HL127682 · NHLBI · UNIVERSITY OF KENTUCKY · PI BERRON, BRADLEY JOSEPH · 2015 to 2018
$2.3M
Lysophosphatidic Acid Mediates Cardiac Inflammation After Acute InfarctionR01HL138488 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ABDEL-LATIF, AHMED · 2017 to 2021
$1.9M
Role of Bioactive Lipids in Stem Cell Mobilization and Homing in Cardiac IschemiaR56HL124266 · NHLBI · UNIVERSITY OF KENTUCKY · PI ABDEL-LATIF, AHMED · 2014 to 2014
$375k
NHLBI NIH HHS R01 HL127682NHLBI NIH HHS R01 HL138488NHLBI NIH HHS R56 HL124266NIGMS NIH HHS P20 GM103527NIGMS NIH HHS P30 GM127211
6 · The paper itself

Abstract

introductionAcute myocardial infarction (AMI) and resulting cardiac damage and heart failure are leading causes of morbidity and mortality worldwide. Multiple studies have examined the utility of CD34

methodsmhPBCs were processed following acquisition using FACS or MACS according to clinically established protocols. Cell viability, CD34 cell purity and characterization of surface marker expression were assessed using a flow cytometer. For in vivo characterization of cardiac repair, we conducted LAD ligation surgery on 8-10 weeks female NOD/SCID mice followed by intramyocardial transplantation of unselected mhPBMNCs, FACS or MACS enriched CD34

resultsBoth MACS and FACS isolation methods achieved high purity rates, viability, and enrichment of CD34

conclusionCell based therapy using enriched CD34

Indexed as

Flow CytometryMagnetic PhenomenaAnimalsAntigens, CD34Cell SeparationCicatrixFemaleFibrosisHumansImmunomodulationInflammationMice, Inbred NODMice, SCIDMyocardial InfarctionVentricular RemodelingAntigens, CD34AngiogenesisCD34 cellsFluorescence activated cell sorting (FACS)InflammationMagnetic beads cell sorting (MACS)Myocardial infarction

Identifiers

PMID31953639
PMCPMC7469922
OpenAlexW3000496715

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.