Evidence map›Paper›PMID 31953562›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2020

lncRNA H19 sponging miR-93 to regulate inflammation in retinal epithelial cells under hyperglycemia via XBP1s.

Rong Luo, Fan Xiao, Pei Wang, Yu-Xiang Hu

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 40 citations in OpenAlex.

  1. Trial
  2. International journal of molecular sciences · 2026
    Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Molecular Mechanism Underlying Role of the XBP1s in Cardiovascular Diseases.Journal of cardiovascular development and disease · 2022
    Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Review
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Rong LuoDepartment of Ophthalmology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, No. 92, Aiguo Road, Donghu District, Nanchang, 330006, Jiangx, People's Republic of China. luorongs57@163.com.ORCID http://orcid.org/0000-0001-6487-2893
Fan XiaoDepartment of Ophthalmology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, No. 92, Aiguo Road, Donghu District, Nanchang, 330006, Jiangx, People's Republic of China.
Pei WangDepartment of Ophthalmology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, No. 92, Aiguo Road, Donghu District, Nanchang, 330006, Jiangx, People's Republic of China.
Yu-Xiang HuDepartment of Ophthalmology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, No. 92, Aiguo Road, Donghu District, Nanchang, 330006, Jiangx, People's Republic of China.
Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveDiabetic retinopathy (DR) is a major complication of both type 1 and type 2 diabetes. Recently, inflammation was found to play an important role in DR pathogenesis. But the mechanism has not been fully understood.

methodsARPE-19 cells were cultured under normal condition and high-glucose condition, then the expressions of miR-93, XBP1s and lncRNA H19 were measured using RT-qPCR or western blots. Besides, the mRNA level of eIF2α and GRP78 and protein level of p-eIF2α and GRP78 were measured by RT-qPCR or western blots. In addition, RT-qPCR and ELISA were adopted to examine the expression and secretion of cytokine factors in these conditions. Dual-luciferase reporter gene assay was used to elucidate the binding and regulation among XBP1s, miR-93 and H19. RNA immunoprecipitation was also performed to verify the interaction between H19 and miR-93. The expressions of DNAJC3 and DNAJB9, the downstream targets of XBP1s, were detected by RT-qPCR.

resultsWe identified that H19 and XBP1s were down-regulated in ARPE-19 cells under high-glucose condition, while miR-93 was up-regulated. ER stress inducers TM and IRE1 inhibitor STF-083010 were adopted and data suggest that ER stress could be induced during high-glucose treatment. In addition, the altered expressions of miR-93, XBP1s and H19 might mediate the increased level of pro-inflammatory cytokines. Furthermore, miR-93 interacted with either lncRNA H19 or XBP1s then modulating the inflammatory processes.

conclusionsH19 played an important role in regulating inflammatory processes in retinal endothelial cells under high-glucose condition through modulating miR-93/XBP1s axis.

Indexed as

CytokinesDiabetic RetinopathyEndoplasmic Reticulum Chaperone BiPEnzyme-Linked Immunosorbent AssayEpithelial CellsEukaryotic Initiation Factor-2Heat-Shock ProteinsHSP40 Heat-Shock ProteinsHumansHyperglycemiaImmunoprecipitationInflammationMembrane ProteinsMicroRNAsMolecular ChaperonesRetinaCytokinesDNAJB9 protein, humanDNAJC3 protein, humanEndoplasmic Reticulum Chaperone BiPEukaryotic Initiation Factor-2H19 long non-coding RNAHeat-Shock ProteinsHSP40 Heat-Shock ProteinsHSPA5 protein, humanMembrane ProteinsMicroRNAsMIRN93 microRNA, humanMolecular ChaperonesRNA, Long NoncodingX-Box Binding Protein 1XBP1 protein, humanDiabetic retinopathyH19InflammationLong non-coding RNAMicroRNAmiR-93

Identifiers

PMID31953562
OpenAlexW3000204277

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.