Evidence map›Paper›PMID 31953261›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2020

Constructing a yeast to express the largest cellulosome complex on the cell surface.

Marimuthu Anandharaj, Yu-Ju Lin, Rizwana Parveen Rani, Eswar Kumar Nadendla, Meng-Chiao Ho, Chieh-Chen Huang, Jan-Fang Cheng, Jui-Jen Chang, Wen-Hsiung Li

Open access · bronzeAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 77 citations in OpenAlex.

  1. Article
  2. Discovery and functional characterization of endoglucanases fromApplied and environmental microbiology · 2026
    Article
  3. Article
  4. Creating a large designer cellulosome in yeast to boost ethanol production.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024
    Review
  13. Review
  14. Review
  15. Article
  16. BioprospectingFrontiers in bioengineering and biotechnology · 2022
    Review
  17. Article
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Marimuthu AnandharajBiodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan.ORCID 0000-0002-7820-2038
Yu-Ju LinBiodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan.
Rizwana Parveen RaniBiodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan.ORCID 0000-0003-4646-4196
Eswar Kumar NadendlaInstitute of Biological Chemistry, Academia Sinica, 11529 Taipei, Taiwan.
Meng-Chiao HoInstitute of Biological Chemistry, Academia Sinica, 11529 Taipei, Taiwan.ORCID 0000-0002-5424-4524
Chieh-Chen HuangDepartment of Life Sciences, National Chung Hsing University, 40227 Taichung, Taiwan.
Jan-Fang ChengDepartment of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, CA 94598.
Jui-Jen ChangDepartment of Medical Research, China Medical University Hospital, China Medical University, 402 Taichung, Taiwan; lancecjj@gmail.com whli@uchicago.edu.
Wen-Hsiung LiBiodiversity Research Center, Academia Sinica, 11529 Taipei, Taiwan; lancecjj@gmail.com whli@uchicago.edu.
National Chung Hsing University · TWBiodiversity Research Center, Academia Sinica · TWInstitute of Biological Chemistry, Academia Sinica · TWChina Medical University · TWLawrence Berkeley National Laboratory · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cellulosomes, which are multienzyme complexes from anaerobic bacteria, are considered nature's finest cellulolytic machinery. Thus, constructing a cellulosome in an industrial yeast has long been a goal pursued by scientists. However, it remains highly challenging due to the size and complexity of cellulosomal genes. Here, we overcame the difficulties by synthesizing the

Indexed as

Acetivibrio thermocellusBacterial Outer Membrane ProteinsBacterial Proteinsbeta-GlucosidaseCell Cycle ProteinsCell MembraneCellulaseCelluloseCellulosomesChromosomal Proteins, Non-HistoneChromosomesCohesinsEthanolFungal ProteinsKluyveromycesMembrane ProteinsBacterial Outer Membrane ProteinsBacterial Proteinsbeta-GlucosidaseCell Cycle ProteinsCellulaseCelluloseChromosomal Proteins, Non-HistoneCipA protein, ClostridiumCohesinsEthanolFungal ProteinsMembrane ProteinsOlpB protein, Clostridium thermocellumRecombinant Fusion Proteinsanchoring proteincellulose-binding modulecellulosomescaffoldin protein

Identifiers

PMID31953261
PMCPMC7007581
OpenAlexW3000595343

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.