Evidence map›Paper›PMID 31953152›Full record

ReviewMethods (San Diego, Calif.)2020

Cutting edge approaches for rapid characterization of airway exosomes.

Kenneth P Hough, Jessy S Deshane

Open access · greenAbstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kenneth P HoughDivision of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35233, United States.
Jessy S DeshaneDivision of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35233, United States. Electronic address: treena@uab.edu.
University of Alabama at Birmingham · US

Funding

Virology CoreP30AI027767 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI OLAF KUTSCH · 1988 to 2026
$84.1M
Therapeutic Targeting of the Myofibroblast in Fibrotic Lung DiseaseP01HL114470 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI THANNICKAL, VICTOR J. · 2013 to 2022
$17.8M
ROS Modulation of Innate and Adaptive Immunity in RAP30AR048311 · NIAMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MOUNTZ, JOHN D · 2001 to 2017
$9.5M
Project 5 Nano-micro Hybrid Fibrous Materials for Containment Removal and Site RemediationP42ES027723 · NIEHS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI LUNGU, CLAUDIU T · 2020 to 2024
$6.7M
Environmental Cadmium and COPDR01ES029981 · NIEHS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ANTONY, VEENA B. · 2018 to 2022
$2.5M
Myeloid-Derived Regulatory Cells in AsthmaR01HL128502 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI DESHANE, JESSY SATYADAS · 2016 to 2020
$1.8M
NHLBI NIH HHS P01 HL114470NHLBI NIH HHS R01 HL128502NIAID NIH HHS P30 AI027767NIAMS NIH HHS P30 AR048311NIEHS NIH HHS P42 ES027723NIEHS NIH HHS R01 ES029981
6 · The paper itself

Abstract

Exosomes have been described as promising biomarkers for understanding disease progression and prognosis. These lipid membrane nanoparticles derived from airway cells have been shown to have immunomodulatory effects, such as driving inflammatory responses in asthma. These emerging evidences demonstrating an important pathophysiological role of exosomes warrants the development of novel approaches for isolation and rapid characterization of exosomes, which would be applicable for both translational and clinical studies. In this review article, we describe two methods of rapid exosomes characterization: (1) imaging flow cytometry using ImageStream; and (2) conventional flow cytometry using the BD Symphony A5 platform. We also explore sorting of exosomes using the BD Aria.

Indexed as

SoftwareAntigens, CDAsthmaBiomarkersBronchoalveolar Lavage FluidCentrifugation, Density GradientExosomesFlow CytometryGene ExpressionHLA-DR AntigensHumansImage Processing, Computer-AssistedLungUltracentrifugationAntigens, CDBiomarkersHLA-DR AntigensExosome characterizationExosomesExtracellular vesiclesFlow cytometryImaging flow cytometryNanoparticles

Identifiers

PMID31953152
PMCPMC7198350
OpenAlexW3000452788

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.