ArticleDental materials : official publication of the Academy of Dental Materials2020
Optimization of a real-time high-throughput assay for assessment of Streptococcus mutans metabolism and screening of antibacterial dental adhesives.
Article in Dental materials : official publication of the Academy of Dental Materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Co-doped titanium dioxide nanoparticles decrease the cytotoxicity of experimental hydrogen peroxide gels for in-office tooth bleaching.Clinical oral investigations · 2024Article
- Optimization of an ultra-bright real-time high-throughput renilla luciferase assay for antibacterial assessment of Streptococcus mutans biofilms.Dental materials : official publication of the Academy of Dental Materials · 2024Article
- Understanding the Influence of Rheology on Biofilm Adhesion and Implication for Food Safety.International journal of food science · 2024Review
- Shear Bond Strength and Color Stability of Novel Antibacterial Nanofilled Dental Adhesive Resins.Nanomaterials (Basel, Switzerland) · 2022Article
- Characterization of Experimental Nanoparticulated Dental Adhesive Resins with Long-Term Antibacterial Properties.Nanomaterials (Basel, Switzerland) · 2022Article
- Novel Experimental In-Office Bleaching Gels Containing Co-Doped Titanium Dioxide Nanoparticles.Nanomaterials (Basel, Switzerland) · 2022Article
- Antibacterial efficacy of non-thermal atmospheric plasma against Streptococcus mutans biofilm grown on the surfaces of restorative resin composites.Scientific reports · 2021Article
- Assessment of Streptococcus mutans biofilms on orthodontic adhesives over 7 days.American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics · 2021Article
- Bibliometric Analysis of Literature Published on Antibacterial Dental Adhesive from 1996-2020.Polymers · 2020Review
- Interaction between the Oral Microbiome and Dental Composite Biomaterials: Where We Are and Where We Should Go.Journal of dental research · 2020Article
- Sorption, solubility and cytotoxicity of novel antibacterial nanofilled dental adhesive resins.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveThe present work shows the optimization of a high-throughput bioluminescence assay to assess the metabolism of intact Streptococcus mutans biofilms and its utility as a screening method for nanofilled antibacterial dental materials.
methodsThe assay was optimized by monitoring changes in bioluminescence mediated by variation of the experimental parameters investigated (growth media and sucrose concentration, inoculum:D-Luciferin ratio, dilution factor, inoculum volume, luminescence wavelength, replicate and luciferase metabolic activity). Confocal microscopy was then used to demonstrate the impact of biofilm growth conditions on the 3-D distribution of extracellular polymeric substance (EPS) within Streptococcus mutans biofilms and its implications as confounding factors in high-throughput studies (HTS).
resultsRelative Luminescence Unit (RLU) values from the HTS optimization were analyzed by multivariate ANOVA (α = 0.05) and coefficients of variation, whereas data from 3-D structural parameters and RLU values of biofilms grown on experimental antibacterial dental adhesive resins were analyzed using General Linear Models and Student-Newman-Keuls post hoc tests (α = 0.05). Confocal microscopy demonstrated that biofilm growth conditions significantly influenced the quantity and distribution of EPS within the 3-D structures of the biofilms. An optimized HTS bioluminescence assay was developed and its applicability as a screening method in dentistry was demonstrated using nanofilled experimental antibacterial dental adhesive resins. SIGNIFICANCE: The present study is anticipated to positively impact the direction of future biofilm research in dentistry, because it offers fundamental information for the design of metabolic-based assays, increases the current levels of standardization and reproducibility while offering a tool to decrease intra-study variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.