Evidence map›Paper›PMID 31952315›Full record

ArticleInternational journal of molecular sciences2020

Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility.

Lina Chen, Xiang Luo, Xuanwen Yang, Dan Jing, Xiaocong Xia, Haoxian Li, Krishna Poudel, Shangyin Cao

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lina ChenZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Xiang LuoZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Xuanwen YangZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Dan JingZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Xiaocong XiaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Haoxian LiZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Krishna PoudelZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.
Shangyin CaoZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009 Zhengzhou, China.

Funding

The Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences CAAS-ASTIP-2020-ZFRIThe Key Project of the National Science and Technology Basic Work of China 2012FY110100
6 · The paper itself

Abstract

Female sterility is a key factor restricting plant reproduction. Our previous studies have revealed that pomegranate female sterility mainly arose from the abnormality of ovule development. MicroRNAs (miRNAs) play important roles in ovule development. However, little is known about the roles of miRNAs in female sterility. In this study, a combined high-throughput sequencing approach was used to investigate the miRNAs and their targeted transcripts involved in female development. A total of 103 conserved and 58 novel miRNAs were identified. Comparative profiling indicated that the expression of 43 known miRNAs and 14 novel miRNAs were differentially expressed between functional male flowers (FMFs) and bisexual flowers (BFs), 30 known miRNAs and nine novel miRNAs showed significant differences among different stages of BFs, and 20 known miRNAs and 18 novel miRNAs exhibited remarkable expression differences among different stages of FMFs. Gene ontology (GO) analyses of 144 predicted targets of differentially expressed miRNAs indicated that the "reproduction process" and "floral whorl development" processes were significantly enriched. The miRNA-mRNA interaction analyses revealed six pairs of candidate miRNAs and their targets associated with female sterility. Interestingly, pg-miR166a-3p was accumulated, whereas its predicted targets (

Indexed as

Cluster AnalysisFlowersGene Expression ProfilingGene Expression Regulation, PlantGene OntologyGene Regulatory NetworksGenes, PlantHigh-Throughput Nucleotide SequencingMicroRNAsOvulePlant InfertilityPomegranateReproductionRNA, MessengerRNA, PlantRNA, Small UntranslatedMicroRNAsRNA, MessengerRNA, PlantRNA, Small Untranslatedandromonoecybisexual flowersfunctional male flowershigh-throughput sequencingovulereproduction process

Identifiers

PMID31952315
PMCPMC7013964

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.