ArticleViruses2020
An RNA Thermometer Activity of the West Nile Virus Genomic 3'-Terminal Stem-Loop Element Modulates Viral Replication Efficiency during Host Switching.
Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- Cellular hnRNP D promotes influenza A virus replication by inhibiting TBK1-IRF3-mediated innate immune response.Journal of virology · 2026Article
- The SL-I structural element of the 3' UTR region of West Nile virus participates in the regulation of viral translation.The Journal of general virology · 2026Article
- Thermal adaptation inProceedings. Biological sciences · 2025Article
- A conserved opal termination codon optimizes a temperature-dependent trade-off between protein production and processing in alphaviruses.Science advances · 2025Article
- Genomic Differences Between Nervous Necrosis Virus (NNV) Reassortants Isolated From Wild and Farmed Fish: Implications for Viral Fitness, Temperature Adaptation and Virulence.Transboundary and emerging diseases · 2025Article
- Structure of Essential RNA Regulatory Elements in the West Nile Virus 3'-Terminal Stem Loop.Journal of molecular biology · 2024Article
- Distinct chikungunya virus polymerase palm subdomains contribute to viral protein accumulation and virion production.PLoS pathogens · 2024Article
- The Flavivirus Non-Structural Protein 5 (NS5): Structure, Functions, and Targeting for Development of Vaccines and Therapeutics.Vaccines · 2024Review
- The West Nile virus genome harbors essential riboregulatory elements with conserved and host-specific functional roles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Robo-Therm, a pipeline to RNA thermometer discovery and validation.RNA (New York, N.Y.) · 2024Article
- Review
- The myriad roles of RNA structure in the flavivirus life cycle.RNA biology · 2024Review
- Evaluation of NS4A, NS4B, NS5 and 3'UTR Genetic Determinants of WNV Lineage 1 Virulence in Birds and Mammals.Viruses · 2023Article
- Extra- and intranuclear heat perception and triggering mechanisms in plants.Frontiers in plant science · 2023Review
- Review
- Temperate Conditions Limit Zika Virus Genome Replication.Journal of virology · 2022Article
- Impact of extrinsic incubation temperature on natural selection during Zika virus infection of Aedes aegypti and Aedes albopictus.PLoS pathogens · 2021Article
- Review
- Alternatively spliced isoforms of AUF1 regulate a miRNA-mRNA interaction differentially through their YGG motif.RNA biology · 2021Article
- Information Encoded by the Flavivirus Genomes beyond the Nucleotide Sequence.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 3'-terminal stem-loop (3'SL) of the RNA genome of the flavivirus West Nile (WNV) harbors, in its stem, one of the sequence elements that are required for genome cyclization. As cyclization is a prerequisite for the initiation of viral replication, the 3'SL was proposed to act as a replication silencer. The lower part of the 3'SL is metastable and confers a structural flexibility that may regulate the switch from the linear to the circular conformation of the viral RNA. In the human system, we previously demonstrated that a cellular RNA-binding protein, AUF1 p45, destabilizes the 3'SL, exposes the cyclization sequence, and thus promotes flaviviral genome cyclization and RNA replication. By investigating mutant RNAs with increased 3'SL stabilities, we showed the specific conformation of the metastable element to be a critical determinant of the helix-destabilizing RNA chaperone activity of AUF1 p45 and of the precision and efficiency of the AUF1 p45-supported initiation of RNA replication. Studies of stability-increasing mutant WNV replicons in human and mosquito cells revealed that the cultivation temperature considerably affected the replication efficiencies of the viral RNA variants and demonstrated the silencing effect of the 3'SL to be temperature dependent. Furthermore, we identified and characterized mosquito proteins displaying similar activities as AUF1 p45. However, as the RNA remodeling activities of the mosquito proteins were found to be considerably lower than those of the human protein, a potential cell protein-mediated destabilization of the 3'SL was suggested to be less efficient in mosquito cells. In summary, our data support a model in which the 3'SL acts as an RNA thermometer that modulates flavivirus replication during host switching.
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