ArticleMicromachines2020
Improving Single-Cell Encapsulation Efficiency and Reliability through Neutral Buoyancy of Suspension.
Article in Micromachines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Integrated Microfluidic Chip Enabling Preparation and Immobilization of Cell-Laden Microspheres, and Microsphere-Based Cell Culture and Analysis.Biosensors · 2026Article
- Two-Step Acoustic Cell Separation Based on Cell Size and Acoustic Impedance─toward Isolation of Viable Circulating Tumor Cells.Analytical chemistry · 2025Article
- Periodic Flows in Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2024Review
- Magnetic Stirring Device for Limiting the Sedimentation of Cells inside Microfluidic Devices.Sensors (Basel, Switzerland) · 2024Article
- Unveiling the Potential of Single-Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives.Small science · 2024Article
- Single-Cell Microarray Chip with Inverse-Tapered Wells to Maintain High Ratio of Cell Trapping.Micromachines · 2023Article
- Upgraded User-Friendly Image-Activated Microfluidic Cell Sorter Using an Optimized and Fast Deep Learning Algorithm.Micromachines · 2022Article
- Multiplexed microfluidic chip for cell co-culture.The Analyst · 2022Article
- Single-Cell Infection of Influenza A Virus Using Drop-Based Microfluidics.Microbiology spectrum · 2022Article
- Deep learning detector for high precision monitoring of cell encapsulation statistics in microfluidic droplets.Lab on a chip · 2022Article
- Microencapsulation-based cell therapies.Cellular and molecular life sciences : CMLS · 2022Review
- Single-Cell Microgels for Diagnostics and Therapeutics.Advanced functional materials · 2021Article
- doubletD: detecting doublets in single-cell DNA sequencing data.Bioinformatics (Oxford, England) · 2021Article
- Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield.Frontiers in chemistry · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Single-cell analysis is of critical importance in revealing cell-to-cell heterogeneity by characterizing individual cells and identifying minority sub-populations of interest. Droplet-based microfluidics has been widely used in the past decade to achieve high-throughput single-cell analysis. However, to maximize the proportion of single-cell emulsification is challenging due to cell sedimentation and aggregation. The purpose of this study was to investigate the influence of single-cell encapsulation and incubation through the use of neutral buoyancy. As a proof of concept, OptiPrep™ was used to create neutrally buoyant cell suspensions of THP-1, a human monocytic leukemia cell line, for single-cell encapsulation and incubation. We found that using a neutrally buoyant suspension greatly increased the efficiency of single-cell encapsulation in microdroplets and eliminated unnecessary cell loss. Moreover, the presence of OptiPrep™ was shown to not affect cellular viability. This method significantly improved the effectiveness of single-cell study in a non-toxic environment and is expected to broadly facilitate single-cell analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.