Evidence map›Paper›PMID 31951864›Full record

ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2020

Sensitivity boosts by the CPMAS CryoProbe for challenging biological assemblies.

Alia Hassan, Caitlin M Quinn, Jochem Struppe, Ivan V Sergeyev, Chunting Zhang, Changmiao Guo, Brent Runge, Theint Theint, Hanh H Dao, Christopher P Jaroniec and 8 more

Open access · greenAbstract read
In one paragraph

Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 81 citations in OpenAlex.

  1. Article
  2. NMR crystallography reveals active-site protonation states of Toho-1 β-lactamase in complex with avibactam.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Live Metabolomics with NMR.Analytical chemistry · 2026
    Review
  5. RSC advances · 2026
    Review
  6. Aducanumab binding to AβProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. Review
  8. Article
  9. MAS Cryoprobe Enhances Solid-State NMR Signals of α-Synuclein Fibrils.bioRxiv : the preprint server for biology · 2025
    Article
  10. Aducanumab Binding to AβbioRxiv : the preprint server for biology · 2025
    Article
  11. Article
  12. bioRxiv : the preprint server for biology · 2025
    Article
  13. High-qualitySolid state nuclear magnetic resonance · 2025
    Article
  14. Article
  15. Probe design for high sensitivity proton-detected solid-state NMR.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2025
    Article
  16. Article
  17. Review
  18. p14Nature communications · 2024
    Article
  19. High-efficiency low-powerJournal of magnetic resonance (San Diego, Calif. : 1997) · 2024
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 6 institutions in 3 countries.

Alia HassanBruker Biospin Corporation, Fällanden, Switzerland. Electronic address: Alia.Hassan@bruker.com.
Caitlin M QuinnDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.
Jochem StruppeBruker Biospin Corporation, 15 Fortune Drive, Billerica, MA, United States.
Ivan V SergeyevBruker Biospin Corporation, 15 Fortune Drive, Billerica, MA, United States.
Chunting ZhangDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.
Changmiao GuoDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.
Brent RungeDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.
Theint TheintDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, United States.
Hanh H DaoDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, United States.
Christopher P JaroniecDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, United States.
Mélanie BerbonCNRS, CBMN, UMR5248, University of Bordeaux, F-33600 Pessac, France.
Alons LendsCNRS, CBMN, UMR5248, University of Bordeaux, F-33600 Pessac, France.
Birgit HabensteinCNRS, CBMN, UMR5248, University of Bordeaux, F-33600 Pessac, France.
Antoine LoquetCNRS, CBMN, UMR5248, University of Bordeaux, F-33600 Pessac, France.
Rainer KuemmerleBruker Biospin Corporation, Fällanden, Switzerland.
Barbara PerroneBruker Biospin Corporation, Fällanden, Switzerland. Electronic address: barbara.perrone@bruker.com.
Angela M GronenbornPittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States; Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Ave., Pittsburgh, PA, United States. Electronic address: amg100@pitt.edu.
Tatyana PolenovaDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States. Electronic address: tpolenov@udel.edu.
Bruker (United States) · USCentre National de la Recherche Scientifique · FRThe Ohio State University · USUniversity of Delaware · USUniversity of Pittsburgh · USBruker (Switzerland) · CH

Funding

Technology Development 2: MAS NMR and dynamic nuclear polarization for HIV-1 structural biologyP50AI150481 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GRONENBORN, ANGELA M. · 2019 to 2021
$14.1M
Pilot Research Subproject ProgramP30GM110758 · NIGMS · UNIVERSITY OF DELAWARE · PI PATEL, SANDEEP · 2014 to 2018
$5.8M
Structural determinants of amyloid strain heterogeneity in distinct phenotypes of Alzheimer's diseaseRF1AG061797 · NIA · CASE WESTERN RESERVE UNIVERSITY · PI JARONIEC, CHRISTOPHER P, SAFAR, JIRI G. · 2018 to 2021
$4.4M
Molecular Mechanisms of Prion and Amyloid PropagationR01GM094357 · NIGMS · OHIO STATE UNIVERSITY · PI JARONIEC, CHRISTOPHER P · 2011 to 2022
$3.2M
Structural and dynamic studies of histone tails in chromatin by magnetic resonance spectroscopyR01GM123743 · NIGMS · OHIO STATE UNIVERSITY · PI JARONIEC, CHRISTOPHER P, POIRIER, MICHAEL GUY · 2017 to 2018
$658k
NIAID NIH HHS P50 AI150481NIA NIH HHS RF1 AG061797NIGMS NIH HHS P30 GM110758NIGMS NIH HHS R01 GM094357NIGMS NIH HHS R01 GM123743
6 · The paper itself

Abstract

Despite breakthroughs in MAS NMR hardware and experimental methodologies, sensitivity remains a major challenge for large and complex biological systems. Here, we report that 3-4 fold higher sensitivities can be obtained in heteronuclear-detected experiments, using a novel HCN CPMAS probe, where the sample coil and the electronics operate at cryogenic temperatures, while the sample is maintained at ambient temperatures (BioSolids CryoProbe™). Such intensity enhancements permit recording 2D and 3D experiments that are otherwise time-prohibitive, such as 2D

Indexed as

Capsid ProteinsCarbonCold TemperatureEscherichia coliFungiHIV-1HumansHydrogen CyanideIndicators and ReagentsKinesinsMicroscopy, Electron, TransmissionMicrotubulesNuclear Magnetic Resonance, BiomolecularPrion ProteinsSensitivity and SpecificityTemperatureCapsid ProteinsCarbonHydrogen CyanideIndicators and ReagentsKIF5B protein, humanKinesinsPrion ProteinsBiological assembliesCryoProbeMagic angle spinningMAS NMR

Identifiers

PMID31951864
PMCPMC7060763
OpenAlexW2996950566

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.