ArticleJournal of assisted reproduction and genetics2020
The impact of culture conditions on blastocyst formation and aneuploidy rates: a comparison between single-step and sequential media in a large academic practice.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Effect of sequential versus single-step culture medium on IVF treatments, including embryo and clinical outcomes: a prospective randomized study.Archives of gynecology and obstetrics · 2022Trial
- A comparison between the assisted reproductive technology outcomes of using two media during sperm microinjection: A bicarbonate medium (one-step) vs. a HEPES-buffered medium (SynVitro Flush).Clinical and experimental reproductive medicine · 2026Article
- Sequencing platforms influence segmental aneuploidy and cumulative live birth rate: A single-center cohort study.Journal of assisted reproduction and genetics · 2026Article
- Inappropriate range of fasting plasma glucose may affect embryo euploidy negatively: a retrospective cohort study.BMC women's health · 2025Article
- Structural rearrangements affect blastocyst development.Archives of gynecology and obstetrics · 2025Article
- Advances in supplementation strategies for enhancing bovine in vitro embryo production: a review of growth factors and antioxidants.Journal of assisted reproduction and genetics · 2025Review
- Independent predictors and thresholds of in vitro fertilization outcomes in patients with diminished ovarian reserve.Scientific reports · 2025Article
- Clinical application of polar body-based preimplantation genetic testing for maternal mutations in women with a limited number of oocytes.Orphanet journal of rare diseases · 2025Article
- A cycle-based model to predict no usable blastocyst formation following cycles of in vitro fertilization in patients with normal ovarian reserve.Reproductive biology and endocrinology : RB&E · 2025Article
- Predictive Factors for the Formation of Viable Embryos in Subfertile Patients with Diminished Ovarian Reserve: A Clinical Prediction Study.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Preimplantation genetic testing for aneuploidy: predictive embryonic factors.Journal of assisted reproduction and genetics · 2024Article
- Evolution of Minimally Invasive and Non-Invasive Preimplantation Genetic Testing: An Overview.Journal of clinical medicine · 2024Review
- Embryo Culture Medium Has No Impact on Mosaicism Rates: a Sibling Oocyte Study.Reproductive sciences (Thousand Oaks, Calif.) · 2023Observational
- Protodioscin protects porcine oocytes against H2O2-induced oxidative stress during in vitro maturation.Animal bioscience · 2023Article
- The influence of day 3 embryo cell number on the clinical pregnancy and live birth rates of day 5 single blastocyst transfer from frozen embryo transfer cycles.BMC pregnancy and childbirth · 2022Article
- Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used.Biological research · 2022Article
- Is Blastocyst Culture Responsible for Higher Pregnancy Rates? A Critical Analysis of the Day of Optimal Embryo Transfer and Embryo Quality.JBRA assisted reproduction · 2022Article
- Does Post-Warming Extended Culture Duration Affect the Clinical and Obstetric Outcomes of Patients of Advanced Maternal Age? A Single-Center Study.Journal of Korean medical science · 2022Article
- Euploidy rates are not affected when embryos are cultured in a continuous (CCM) or sequential culture medium (SCM): a sibling oocyte study.Journal of assisted reproduction and genetics · 2021Observational
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
purposeTo compare a single-step medium with a sequential medium on human blastocyst development rates, aneuploidy rates, and clinical outcomes.
methodsRetrospective cohort study of IVF cycles that used Sage advantage sequential medium (n = 347) and uninterrupted Sage 1-step medium (n = 519) from July 1, 2016, to December 31, 2017, in an academic fertility center. Main outcome measures are blastocyst formation rates per two-pronuclear (2PN) oocyte and aneuploidy rates per biopsy.
resultsOf all IVF cycles, single-step medium yielded higher blastocyst formation rate (51.7% vs 43.4%) but higher aneuploidy rate (54.0% vs 45.8%) compared with sequential medium. When stratified by maternal age, women under age 38 had no difference in blastocyst formation (52.2% vs 50.2%) but a higher aneuploidy rate (44.5% vs 36.4%) resulting in a lower number of euploid blastocysts per cycle (2.6 vs 3.3) when using single-step medium compared to sequential medium. In cycles used single-step medium, patients ≥ age 38 had higher blastocyst rate (48.0% vs 33.6%), but no difference in aneuploidy rate (68.8% vs 66.0%) or number of euploid embryos (0.8 vs 1.1). For patients reaching euploid embryo transfer, there was no difference in clinical pregnancy rates, miscarriage rates, or live birth rates between two culture media systems.
conclusionsOur study demonstrates an increase in aneuploidy in young women whose embryos were cultured in a single-step medium compared to sequential medium. This study highlights the importance of culture conditions on embryo ploidy and the need to stratify by patient age when examining the impact of culture conditions on overall cycle potential.
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