Evidence map›Paper›PMID 31950325›Full record

ReviewMolecular biology reports2020

The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins' study.

Diana Moreira, Ana Marta Pereira, Ana Lúcia Lopes, Sílvia Coimbra

Open access · hybridAbstract readReview
PubMed Publisher
In one paragraph

Review in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
  7. Cracking the "Sugar Code": A Snapshot ofFrontiers in plant science · 2021
    Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 3 countries.

Diana MoreiraDepartamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal.
Ana Marta PereiraDipartimento di Bioscienze, Università Degli Studi di Milano, Milano, Italy.
Ana Lúcia LopesDepartamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal.
Sílvia CoimbraDepartamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal. scoimbra@fc.up.pt.ORCID http://orcid.org/0000-0001-9421-2538
Universidade do Porto · PTUniversity of Milan · IT

Funding

Fundação para a Ciência e a Tecnologia SFRH/BD/115960/2016Fundação para a Ciência e a Tecnologia SFRH/BD/143557/2019H2020 Marie Skłodowska-Curie Actions 753328Horizon 2020 690946
6 · The paper itself

Abstract

Arabinogalactan Proteins (AGPs) are hydroxyproline-rich proteins containing a high proportion of carbohydrates, widely spread in the plant kingdom. AGPs have been suggested to play important roles in plant development processes, especially in sexual plant reproduction. Nevertheless, the functions of a large number of these molecules, remains to be discovered. In this review, we discuss two revolutionary genetic techniques that are able to decode the roles of these glycoproteins in an easy and efficient way. The RNA interference is a frequently technique used in plant biology that promotes genes silencing. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (CRISPR/Cas9), emerged a few years ago as a revolutionary genome-editing technique that has allowed null mutants to be obtained in a wide variety of organisms, including plants. The two techniques have some differences between them and depending on the research objective, these may work as advantage or disadvantage. In the present work, we propose the use of the two techniques to obtain AGP mutants easily and quickly, helping to unravel the role of AGPs, surely a great asset for the future.

Indexed as

CRISPR-Cas SystemsGene EditingGene Expression RegulationRNA InterferenceAnimalsGene SilencingGene TargetingHumansMucoproteinsPlant ProteinsResearchRNA, Small Interferingarabinogalactan proteinsMucoproteinsPlant ProteinsRNA, Small InterferingArabinogalactan proteinsCRISPR/Cas9MutantsPlant genome engineeringRNA interference

Identifiers

PMID31950325
OpenAlexW2999859865

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.