ArticleCellular and molecular neurobiology2020
Silencing the Cytoskeleton Protein Iba1 (Ionized Calcium Binding Adapter Protein 1) Interferes with BV2 Microglia Functioning.
Article in Cellular and molecular neurobiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Prenatal and Lactation Exposure to Bisphenol A (BPA) at the Global Mean Daily Intake Level Leads to Sex-Dependent Changes in Microglial State, Gene Expression, and Behavioral Patterns in Newborn and Juvenile Wistar Rat Offspring.Life (Basel, Switzerland) · 2026Article
- Neonatal estradiol influences regional gene expression for early sex-specific inflammatory responses in the 5xFAD mouse model.Biology of sex differences · 2026Article
- Hematopoietic monoamine oxidase A deficiency exacerbates neuroinflammation and demyelination in female but not male mice with experimental autoimmune encephalomyelitis.Frontiers in immunology · 2026Article
- Rotenone-induced oxidative stress triggers peroxisomal alterations in BV-2 microglial cells.Frontiers in cellular neuroscience · 2026Article
- Study on the Mechanism of Acanthopanax senticosus Extract Intervening in the Regulation of Parkinson's Disease by BV2 Microglial Cell-Derived Exosomes.Molecular neurobiology · 2025Article
- Transcription Factor-Based Differentiation of Pluripotent Stem Cells: Overcoming the Traps of Random Neuronal Fate.Biomedicines · 2025Review
- NDR2 Kinase Regulates Microglial Metabolic Adaptation and Inflammatory Response: Critical Role in Glucose-Dependent Functional Plasticity.International journal of molecular sciences · 2025Article
- Review
- New insights into the role of the endoplasmic reticulum in microglia.Neural regeneration research · 2024Article
- Dorsal root ganglion-derived exosomes deteriorate neuropathic pain by activating microglia via the microRNA-16-5p/HECTD1/HSP90 axis.Biological research · 2024Article
- Urolithin A Prevents Sleep-deprivation-induced Neuroinflammation and Mitochondrial Dysfunction in Young and Aged Mice.Molecular neurobiology · 2024Article
- Switching Rat Resident Macrophages from M1 to M2 Phenotype by Iba1 Silencing Has Analgesic Effects in SNL-Induced Neuropathic Pain.International journal of molecular sciences · 2023Article
- Anti-Inflammatory Activity of Synaptamide in the Peripheral Nervous System in a Model of Sciatic Nerve Injury.International journal of molecular sciences · 2023Article
- The Effect of Spironolactone on β-amyloid-Induced Memory Impairment in Male Rats: The Role of Microglial Inhibition.Advanced pharmaceutical bulletin · 2022Article
- Ki20227 aggravates apoptosis, inflammatory response, and oxidative stress after focal cerebral ischemia injury.Neural regeneration research · 2022Article
- Inherent P2X7 Receptors Regulate Macrophage Functions during Inflammatory Diseases.International journal of molecular sciences · 2021Review
- Chronic Alcoholism and HHV-6 Infection Synergistically Promote Neuroinflammatory Microglial Phenotypes in theBiomedicines · 2021Article
- Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration.International journal of molecular sciences · 2021Article
- Mitochondrial Deficits With Neural and Social Damage in Early-Stage Alzheimer's Disease Model Mice.Frontiers in aging neuroscience · 2021Article
- Microglia Specific Drug Targeting Using Natural Products for the Regulation of Redox Imbalance in Neurodegeneration.Frontiers in pharmacology · 2021Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Iba1 (ionized calcium binding adapter protein 1) is a cytoskeleton protein specific only for microglia and macrophages, where it acts as an actin-cross linking protein. Although frequently regarded as a marker of activation, its involvement in cell migration, membrane ruffling, phagocytosis or in microglia remodeling during immunological surveillance of the brain suggest that Iba1 is not a simple cytoskeleton protein, but a signaling molecule involved in specific signaling pathways. In this study we investigated if Iba1 could also represent a drug target, and tested the hypothesis that its specific silencing with customized Iba1-siRNA can modulate microglia functioning. The results showed that Iba1-silenced BV2 microglia migrate less due to reduced proliferation and cell adhesion, while their phagocytic activity and P2x7 functioning was significantly increased. Our data are the proof of concept that Iba1 protein is a new microglia target, which opens a new therapeutic avenue for modulating microglia behavior.
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Registered trials
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