ArticleInternational journal of clinical and experimental pathology2018
Early discovery of disseminated tumor cells during carcinogenesis in a 4NQO-induced mouse model of oral squamous cell carcinoma.
Article in International journal of clinical and experimental pathology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 4 citations in OpenAlex.
- Distant metastasis of oral squamous cell carcinoma: immune escape mechanism and new perspectives on treatment.Discover oncology · 2025Review
- Temporal and spatial characteristics of tumor evolution in a mouse model of oral squamous cell carcinoma.BMC cancer · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
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Abstract
objectivesHeterogeneous cells appear in multiple organs during the same time period as the primary lesion of some tumors is clinically detected. These heterogeneous cells are also known as disseminated tumor cells (DTCs). However, the characteristics of DTCs that disseminate during oral carcinogenesis remain unclear. MATERIALS AND
methodsA mouse 4NQO model of lymph node metastasis in oral squamous cell carcinoma was established. Tissue samples of the tongue, bone marrow and submandibular lymph node were collected. Five stages (stage 0~stage IV) of carcinogenesis in each experimental animal were classified by two pathologists. After immunohistochemical staining of cytokeratin, the DTCs were isolated from bone marrow samples (stage II) by the laser capture microdissection (LCM) technique during oral carcinogenesis. Genomic amplification of bone marrow DTCs was performed, and homozygous deletion of the RB1CC1 gene was analyzed. After confirming the presence of disseminated tumor cells in stage II bone marrow samples, a comprehensive study among various stages of lymph node tissue was conducted using the same method.
resultsDTCs that spread from the primary tumor were discovered in stage II bone marrow samples and in stage I, stage II and stage III submandibular lymph node samples through immunohistochemical staining. These spreading cells had different levels of homozygous exon deletion in the RB1CC1 and TP53 genes.
conclusionEarly spreading of epithelial cells may occur during the carcinogenesis of oral cancer. DTCs of oral carcinoma may show different chromosome aberrations from matched primary tumor cells.
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Identifiers
31949709PMC6962884W3028103866What OpenQuestion holds
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