ArticleInternational journal of clinical and experimental pathology2018
MicroRNA-142a-3p promotes the differentiation of 3T3-L1 preadipocytes by targeting high-mobility group AT-hook 1.
Article in International journal of clinical and experimental pathology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- Extracellular microRNAs in Relation to Weight Loss-A Systematic Review and Meta-Analysis.Non-coding RNA · 2023Review
- An RNAi Screening of Clinically Relevant Transcription Factors Regulating Human Adipogenesis and Adipocyte Metabolism.Endocrinology · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundObesity is characterized by the excess accumulation of adipose tissues, mainly composed of adipocytes. The differentiation of adipocytes is one of the major events in the process of adipogenesis. Among various adipogenic transcription factors, CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferators-activated receptor γ (PPARγ) have been identified as essential regulators of adipocyte differentiation.
methodsRT-qPCR assay was conducted to detect the expression of microRNA-142a-3p (miR-142a-3p), high-mobility group AT-hook 1 (HMGA1) mRNA, C/EBPα mRNA, and PPARγ mRNA. Western blot assay was performed to measure the protein levels of HMGA1, C/EBPα and PPARγ. Bioinformatics analysis and luciferase reporter assay were carried out to explore the interaction between miR-142a-3p and HMGA1.
resultsmiR-142a-3p expression was notably increased and HMGA1 expression was markedly reduced during 3T3-L1 preadipocyte differentiation. Functional analysis revealed that miR-142a-3p overexpression promoted 3T3-L1 preadipocyte differentiation. Further investigations on molecular mechanisms showed that HMGA1 was a target of miR-142a-3p in 3T3-L1 preadipocytes. Moreover, the knockdown of HMGA1 induced 3T3-L1 preadipocyte differentiation. Additionally, HMGA1 silencing abolished miR-142a-3p deficiency-mediated inhibitory effect on 3T3-L1 preadipocyte differentiation.
conclusionMiR-142a-3p overexpression facilitated 3T3-L1 preadipocyte differentiation by targeting HMGA1, highlighting the importance of miR-142a-3p, HMGA1 and the miR-142a-3p/HMGA1 axis in adipogenesis.
Indexed as
Identifiers
31949605PMC6963016W3026461876What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.