GATA4/6 regulate DHH transcription in rat adrenocortical autografts.

Takashi Yoshida, Nae Takizawa, Tadashi Matsuda, Hisao Yamada, Masaaki Kitada, Susumu Tanaka

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Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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0 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Takashi YoshidaDepartment of Anatomy, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan.ORCID http://orcid.org/0000-0003-1119-312X

Nae TakizawaDepartment of Anatomy, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan.

Tadashi MatsudaDepartment of Urology and Andrology, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan.

Hisao YamadaDepartment of Anatomy, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan.

Masaaki KitadaDepartment of Anatomy, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan.

Susumu TanakaDepartment of Anatomy, Kansai Medical University, Hirakata, Osaka, 573-1010, Japan. tanakass@hirakata.kmu.ac.jp.

Kansai Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adrenal cortex autotransplantation with ACTH stimulation may be an alternative therapy for patients with bilateral adrenalectomy to avoid adrenal crisis, but its underlying mechanism has not been elucidated. Previously, we detected Dhh upregulation in rat adrenocortical autografts after transplantation. Here, we investigated potential regulators such as Gata4, Gata6, Sry and Sox9 which affect Dhh transcription in adrenocortical autografts with or without ACTH stimulation. In ACTH-stimulated autografts, Gata4 and Gata6 were downregulated compared to control autografts. This response was linked to rDhh repression. A reporter assay using the upstream region of rDhh and a GATA binding motif revealed that rDhh promoters were significantly upregulated by co-transfection with Gata4 or Gata6 or both. Sry and Sox9 expression in autografts with or without ACTH stimulation were verified by PCR and RNAscope analyses. The ovarian differentiation factors Foxl2 and Rspo1 were also upregulated in the autografts. Gata4 and Gata6 were found to be significant factors in the regulation of rDhh expression and could be associated with adrenocortical autograft maintenance. Gonadal primordia with bipotential testicular and ovarian functions may also be present in these autografts.

Indexed as

Adrenal CortexAdrenocorticotropic HormoneAnimalsGATA4 Transcription FactorGATA6 Transcription FactorHedgehog ProteinsRatsRegenerationTransplantation, AutologousUp-RegulationAdrenocorticotropic HormoneDhh protein, ratGata4 protein, ratGATA4 Transcription FactorGata6 protein, ratGATA6 Transcription FactorHedgehog Proteins

Identifiers

PMID31949236

DOI10.1093/nar/29.9.e45

PMCPMC6965091

OpenAlexW3005048999

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.