ArticleBMC biotechnology2020
Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays.
Article in BMC biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 24 citations in OpenAlex.
- CsWRKY22 positively regulates quercetin biosynthesis by activating the CsFLSd promoter in tea plants (Camellia sinensis).Plant cell reports · 2026Article
- Deciphering cassava brown streak virus infection in cassava through VPg mediated host protein interactions.Archives of virology · 2026Article
- Genome-wide identification of the P5CS gene family in Psammochloa villosa and functional characterization under drought stress.BMC plant biology · 2026Article
- Construction of a Normalized Library and Screening of Transcriptional Regulators of theMicroorganisms · 2026Article
- MeJA-Induced Plant Disease Resistance: A Review.Plants (Basel, Switzerland) · 2026Review
- Ectopic expression of safflower NAC transcription factorGM crops & food · 2025Article
- Construction of Yeast One-Hybrid Library ofBiomolecules · 2025Article
- Host-pathogen protein interaction studies: quality control of cDNA libraries using nanopore sequencing.PloS one · 2025Article
- Construction of Shoot Apical Meristem cDNA Yeast Library ofCurrent issues in molecular biology · 2024Article
- Identification of Transcription Factors of Santalene Synthase Gene Promoters andPlants (Basel, Switzerland) · 2024Article
- P1 of turnip mosaic virus interacts with NOD19 for vigorous infection.Frontiers in microbiology · 2023Article
- Analysis of the role ofFrontiers in plant science · 2023Article
- Construction of yeast one-hybrid library and screening of transcription factors regulating LhMYBSPLATTER expression in Asiatic hybrid lilies (Lilium spp.).BMC plant biology · 2021Article
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5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one- and two-hybrid three-frame cDNA libraries were constructed.
resultsIn this study, a modified cDNA library used for yeast one- and two-hybrid screening was successfully constructed, with a recombinant efficiency of 95%. The lengths of inserted fragments ranged from 750~3000 bp, and the library capacity reached 6 × 10
conclusionThe yeast one- and two-hybrid library of C. faberi provides large amounts of useful information for the functional genomics research in C. faberi, and this method could also be applied to other plants to screen DNA-protein and protein-protein interactions.
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