Evidence map›Paper›PMID 31945109›Full record

ArticlePloS one2020

Centromeres of Cucumis melo L. comprise Cmcent and two novel repeats, CmSat162 and CmSat189.

Agus Budi Setiawan, Chee How Teo, Shinji Kikuchi, Hidenori Sassa, Kenji Kato, Takato Koba

Abstract read
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Genome Studies inInternational journal of molecular sciences · 2024
    Article
  2. Article
  3. Review
  4. Article
  5. The Genomics of Plant Satellite DNA.Progress in molecular and subcellular biology · 2021
    Review
  6. Review
  7. FunctionalFrontiers in plant science · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Agus Budi SetiawanLaboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan.
Chee How TeoCenter for Research in Biotechnology for Agriculture, University of Malaya, Kuala Lumpur, Malaysia.ORCID 0000-0002-8118-2592
Shinji KikuchiLaboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan.
Hidenori SassaLaboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan.
Kenji KatoGraduate School of Environmental and Life Science, Okayama University, Kita-ku, Okayama, Japan.
Takato KobaLaboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan.ORCID 0000-0002-9204-8858

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft genome sequence of melon, two new SatDNAs (CmSat162 and CmSat189) of melon were identified and their chromosomal distributions were confirmed using fluorescence in situ hybridization. DNA probes prepared from these SatDNAs were successfully hybridized to melon somatic and meiotic chromosomes. CmSat162 was located on 12 pairs of melon chromosomes and co-localized with the centromeric repeat, Cmcent, at the centromeric regions. In contrast, CmSat189 was found to be located not only on centromeric regions but also on specific regions of the chromosomes, allowing the characterization of individual chromosomes of melon. It was also shown that these SatDNAs were transcribed in melon. These results suggest that CmSat162 and CmSat189 might have some functions at the centromeric regions.

Indexed as

Repetitive Sequences, Nucleic AcidCentromereCucumis meloDNA, PlantGenome, PlantGenomicsIn Situ Hybridization, FluorescenceTranscription, GeneticDNA, Plant

Identifiers

PMID31945109
PMCPMC6964814

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.