ArticleJournal of animal science2020
Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection1,2.
Article in Journal of animal science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Perspective: from pathogenesis to treatment in veterinary science through organoid disease modelling.Frontiers in veterinary science · 2026Article
- Isolation, characterization, and pathogenic mechanisms of a novelFrontiers in cellular and infection microbiology · 2026Article
- Relevance of porcine intestinal organoids as a surrogate for animal experimentation: application to the investigation of host-virus interactions during porcine coronavirus infection.Veterinary research · 2025Article
- The use of stem cells and organoids for modeling host-microbe interactions in low-biomass tissues.Frontiers in cellular and infection microbiology · 2025Review
- Protocol to establish turkey oviductal organoids as an in vitro model.STAR protocols · 2024Article
- Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.Tissue barriers · 2024Article
- Development and characterization of segment-specific enteroids from the pig small intestine in Matrigel and transwell inserts: insights into susceptibility to porcine epidemic diarrhea Virus.Frontiers in immunology · 2024Article
- Lawsonia intracellularis regulates nuclear factor-κB signalling pathway during infection.PloS one · 2024Article
- Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis.Veterinary research · 2023Article
- Dietary Zinc Supplemented in Organic Form Affects the Expression of Inflammatory Molecules in Swine Intestine.Animals : an open access journal from MDPI · 2023Article
- Evaluation of porcine intestinal organoids as anJournal of veterinary science · 2023Article
- Advances in organoid technology for veterinary disease modeling.Frontiers in veterinary science · 2023Review
- Evaluation of the role of clathrin and bacterial viability in the endocytosis ofFrontiers in veterinary science · 2023Article
- Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans.Frontiers in medical technology · 2022Review
- Robust Three-Dimensional (3D) Expansion of Bovine Intestinal Organoids: An In Vitro Model as a Potential Alternative to an In Vivo System.Animals : an open access journal from MDPI · 2021Article
- Measures Matter-Determining the True Nutri-Physiological Value of Feed Ingredients for Swine.Animals : an open access journal from MDPI · 2021Review
- Organoids: a promising new in vitro platform in livestock and veterinary research.Veterinary research · 2021Review
- Intestinal organoids in farm animals.Veterinary research · 2021Review
- Harness Organoid Models for Virological Studies in Animals: A Cross-Species Perspective.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enteric pathogen Lawsonia intracellularis is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study L. intracellularis pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of L. intracellularis-infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for L. intracellularis infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by L. intracellularis in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and L. intracellularis immunohistochemistry. In this study, we show that L. intracellularis is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.