ArticleAnalytical and bioanalytical chemistry2020
Column bleed in the analysis of highly polar substances: an overlooked aspect in HRMS.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Comparative non-target screening of advanced oxidation process (AOP)-integrated industrial wastewater treatment using RP and mixed-mode chromatography.Analytical and bioanalytical chemistry · 2026Article
- Persistent and Mobile Organic Contaminants: Quantification and Nontarget Analysis in Greater Boston Drinking and Surface Water.ACS environmental Au · 2026Article
- Enhanced industrial wastewater monitoring: method development for non-target screening of highly polar substances using ZIC-HILIC-HRMS.Analytical and bioanalytical chemistry · 2025Article
- Development of an Untargeted LC-MS Metabolomics Method with Postcolumn Infusion for Matrix Effect Monitoring in Plasma and Feces.Journal of the American Society for Mass Spectrometry · 2024Article
- Electrospray ionization mass spectrometry ion suppression/enhancement caused by column bleed for three mixed-mode reversed-phase/anion-exchange high-performance liquid chromatography columns.Rapid communications in mass spectrometry : RCM · 2021Article
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4 authors.
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Abstract
To close the "analytical gap" in the liquid chromatographic (LC) analysis of highly polar substances, two techniques which have been suggested earlier were tested in terms of retention factors and detection limits: hydrophilic interaction liquid chromatography (HILIC) and mixed-mode chromatography (MMC). A substance mix of 55 analytes ranging from logD - 8.2 to 3.4 and 17 different LC columns, also comprising additional reversed-phase columns were used. Contrary to most reversed-phase columns, column bleed has been identified as an important factor, which may cause serious restrictions during high-resolution mass spectrometric detection (HRMS). We found that highly abundant background masses continuously eluting from the columns heavily influence ion transmission to the detector. As a result, the linear dynamic range as well as the sensitivity decreases and thus limits the HRMS applicability of some columns. We therefore recommend a thorough investigation of ion transmission during HRMS method development. This will help to maintain the high potential of HRMS in terms of qualitative and quantitative screening analysis.
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