ArticleParasitology research2020
A modified method for purification of Eimeria tenella sporozoites.
Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- A novel ligand for chicken Toll-like receptor 5 from Eimeria maxima: dihydrolipoyl dehydrogenase drives M2-like polarization and Treg-like responses.Poultry science · 2026Article
- Examination of artificial oocyst excystation methods in the murine coccidium, Eimeria krijgsmanni.The Journal of veterinary medical science · 2025Article
- Comparative analysis of pathogenicity and anticoccidial resistance of field-isolatedFrontiers in veterinary science · 2025Article
- Monocyte-Derived Chicken Macrophages Exposed toMicroorganisms · 2023Article
- Interplay betweenLife (Basel, Switzerland) · 2023Article
- Identification and Characterization ofMicrobiology spectrum · 2021Article
- In vitro infection of Madin-Darby bovine kidney (MDBK) cells with Eimeria acervulina sporozoites: quantitative analysis of parasite cellular invasion and replication using real-time polymerase chain reaction (PCR).Parasitology research · 2021Article
- In Vitro Assessment of Anticoccidials: Methods and Molecules.Animals : an open access journal from MDPI · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coccidiosis is an economically important gastrointestinal disease in domestic fowl. Eimeria species are the causative agents of avian coccidiosis. Current challenges in management and prevention of eimeriosis enhance the need for research in this field. Sporozoite purification is a necessary step for Eimeria spp. in vitro infection models. Current alternatives such as DE-52 anion exchange chromatography and Percoll gradient require time and resources. We present a modified protocol consisting on vacuum filtration of sporozoites using a disposable 5-μL filter. Yield percentages were similar to those reported for Percoll gradient purification. By reducing time and efforts during sporozoite purification, it could be possible to increase resources in other areas of Eimeria studies.
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Registered trials
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