Evidence map›Paper›PMID 31942007›Full record

ArticleScientific reports2020

Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea.

Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Presence of Free-livingMicroorganisms · 2022
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Heekyoung KangDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
Hae-Jin SohnDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
Ga-Eun SeoDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
Gi-Sang SeongDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
A-Jeong HamDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
A-Young ParkDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
Suk-Yul JungDepartment of Biomedical Laboratory Science, Molecular Diagnostics Research Institute, School of Health and Medicine, Namseoul University, Cheonan, 31020, Republic of Korea.
Sang-Eun LeeDivision of Vectors and Parasitic Diseases, Korea Centers for Diseases Control and Prevention, Osong, 363-951, Republic of Korea.
Shin-Hyeong ChoDivision of Vectors and Parasitic Diseases, Korea Centers for Diseases Control and Prevention, Osong, 363-951, Republic of Korea.
Ho-Joon ShinDepartment of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea. hjshin@ajou.ac.kr.
Ajou University · KRKorea Center for Disease Control and Prevention · KRNamseoul University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The free-living amoebae Naegleria spp. and Acanthamoeba spp. exist in the natural environment and are sometimes causal agents of lethal primary amoebic meningoencephalitis (PAM), amoebic keratitis (AK) and granulomatous amebic encephalitis (GAE) in humans, respectively. To ascertain the existence of free-living amoebae in Korea, water samples were collected from the Korean hydrosphere, Namhangang (southern Han River), an active location for water skiing and recreation. Samples underwent two-step filtration and were cultured on non-nutrient agar medium with inactivated E. coli. The remaining samples were subjected to PCR for primarily the 18S small ribosomal RNA gene and gene sequencing. Similarities in 18S rDNA sequences, in comparison with various reference amoebae in GenBank, showed 86~99% homology with N. gruberi, N. philippinensis, N. clarki, A. polyphaga, A. castellannii, and Hartmannella (Vermamoeba) vermiformis. Therefore, this study will be useful for seasonal detection of free-living amoebae from various Korean hydrospheres in future studies.

Indexed as

AmoebaBase SequencePhylogenyRepublic of KoreaRiversRNA, Ribosomal, 18SSequence AlignmentSequence Analysis, DNARNA, Ribosomal, 18S

Identifiers

PMID31942007
PMCPMC6962209
OpenAlexW2999446312

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.