Evidence map›Paper›PMID 31941947›Full record

ArticleScientific reports2020

Real-time monitoring of bacterial growth kinetics in suspensions using laser speckle imaging.

Hadi Loutfi, Fabrice Pellen, Bernard Le Jeune, Roger Lteif, Mireille Kallassy, Guy Le Brun, Marie Abboud

Abstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hadi LoutfiPhysics Department, UR TVA, Faculty of Science, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon. hadi.loutfi@net.usj.edu.lb.
Fabrice PellenLaboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France.
Bernard Le JeuneLaboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France.
Roger LteifChemistry Department, UR TVA, Faculty of Science, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon.
Mireille KallassyFaculty of Science, Biotechnology Laboratory, UR EGP, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon.
Guy Le BrunLaboratoire OPTIMAG, IBSAM, Université de Bretagne Occidentale, 6 avenue Le Gorgeu, C.S. 93837, 29238, Brest Cedex, 3, France. guy.lebrun@univ-brest.fr.
Marie AbboudPhysics Department, UR TVA, Faculty of Science, Saint Joseph University, B.P. 11-514, Riad El Solh Beirut, 1107 2050, Lebanon. marie.abboud@usj.edu.lb.

Funding

Conseil National de la Recherche Scientifique 03-05-17Saint Joseph University USJ-FS109Seventh Framework Programme 2-72-093
6 · The paper itself

Abstract

In microbiology, monitoring the growth of any microorganism in culture is important for studying and optimizing the growth kinetics, the biomass and the metabolite production. In this work, we show that laser speckle imaging is a reliable technique that can be used to perform real-time monitoring of bacteria growth kinetic in liquid culture media. Speckle parameters, specifically speckle grain size and the spatial contrast of the speckle images, and standard analytical parameters (optical density, pH and colony forming units) were measured during the culture of different strains of Bacillus thuringiensis. Our results show that both speckle grain size and spatial contrast decrease with bacterial growth. Furthermore, speckle parameters are sensitive to the fermentation conditions. Statistical analysis revealed a relatively high correlation between speckle and analytical parameters.

Indexed as

AlgorithmsBacillus thuringiensisImage Interpretation, Computer-AssistedLaser-Doppler FlowmetryMonitoring, PhysiologicOptical ImagingSuspensionsSuspensions

Identifiers

PMID31941947
PMCPMC6962325

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.